Nitrofuran (AMOZ) ELISA Test Kit

Nitrofuran (AMOZ) ELISA Test Kit

Catalog No. LSY-10001

This test kit is based on the competitive enzyme immunoassay for the detection of AMOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AMOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AMOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AMOZ in it. This value is compared to the standard curve and the AMOZ concentration is subsequently obtained.

Sensitivity:  0.05 ppb

Incubation Temperature:  25

Incubation Time:  30min15min

Cross-reaction rate

Recovery rate

1)     Micro-well strips: 12 strips with 8 removable wells each

2)     6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb

3)     Enzyme conjugate (7 mL).............................................................. red cap

4)     Antibody working solution (7 mL)................................................. blue cap

5)     Substrate A solution (7 mL)........................................................ white cap

6)     Substrate B solution (7 mL)........................................................ black cap

7)     Stop solution (7 mL)................................................................. yellow cap

8)     20× concentrated washing buffer (40 mL).................................... white cap

9)     2× concentrated redissolving solution (50 mL).................... transparent cap

10)  2-Nitrobenzaldehyde (C7H5NO3)(10 mL)........................................ white cap




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