Dexamethasone ELISA test Kit

Dexamethasone ELISA test Kit Catalog No. LSY-10042 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Dexamethasone in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Dexamethasone in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Dexamethasone antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Dexamethasone in it. This value is compared to the standard curve and the Dexamethasone concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.1ppb Incubation Temperature: 25℃ Incubation Time: 30min ~ 15min Detection limit: Tissue 0.1 ppb Feed 0.2 ppb Milk powder 0.3 ppb Milk 0.2 ppb Honey 0.2 ppb Urine 0.2 ppb Cross-reaction rate: Dexamethasone 100% Recovery rate Tissue, feed 90±25% Milk powder 85±25% Urine, milk, honey…………………………………………………………. 95±25% 3. Components 1) Micro-well strips: 12 strips with 8 removable wells each 2) 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb 3) Enzyme conjugate (7 mL) red cap 4) Antibody working solution (7 mL) blue cap 5) Substrate A solution (7 mL) white cap 6) Substrate B solution (7 mL) black cap 7) Stop solution (7 mL) yellow cap 8) 20× concentrated washing buffer (15 mL) white cap 9) 5× concentrated redissolving solution (110 mL) transparent cap




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