Aflatoxins B1 ELISA test kit

Aflatoxins B1 (AFB1) ELISA Test Kit Catalog No. LSY-10028 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Aflatoxins B1. The coupling antigen is pre-coated on the micro-well stripes. The Aflatoxins B1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Aflatoxins B1 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Aflatoxins B1 in the sample. This value is compared to the standard curve and the Aflatoxins B1 residues is subsequently obtained.2. Technical specifications Sensitivity: 0.03 ppb Incubator temperature: 25℃ Incubator time: 30min~15min Detection limit: Milk (Method 1)…………………………………………………….……...0.15ppb Milk (Method 2)…………………………………………….....…………....0.3ppb Tissue……………………………………………………………………...0.72ppb Feed ……………………………………………………………………..…0.9ppb Urine………………………………………………………………..….….0.06ppb Cross-reaction rate: Aflatoxins B1… ……..100% Recovery rate: Milk……………………………………………………………………….… 90±23% Tissue…………………………………………………………………..…. 85±18% Feed,urine……………………………………………….……………….. 85±21%3. Components 1) Micro-well strips: 12 strips with 8 removable wells each 2) 6× standard solution (1 mL each): 0 ppb, 0.03 ppb, 0.06 ppb, 0.12 ppb, 0.24 ppb ,0.48 ppb 3) Enzyme conjugate (7 mL) red cap 4) Antibody working solution (7 mL) blue cap 5) Substrate A solution (7 mL) white cap 6) Substrate B solution (7 mL) black cap 7) Stop solution (7 mL) yellow cap 8) 20× concentrated washing buffer (40 mL) white cap 9) 2× concentrated redissolving solution (50 mL) transparent cap



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