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Mouse anti-endothelial cell antibody,AECA ELISA Kit

Mouse anti-endothelial cell antibody,AECA ELISA Kit
  • Mouse anti-endothelial cell antibody,AECA ELISA Kit
Produced by: Shanghai Crystal day Biotech Co,Ltd.

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Thiskitusesenzyme-linkedimmunesorbentassay(ELISA)basedontheBiotindoubleantibodysandwichtechnologytoassaytheHumanGhrelin(GHRL).AddGhrelin(GHRL)tothewells,whicharepre-coatedwithGhrelin(GHRL)monoclonalantibodyandthenincubate.Afterthat,addantiGHRLantibodieslabeledwithbiotintounitewithstreptavidin-HRP,whichformsimmunecomplex.Removeunboundenzymesafterincubationandwashing.AddsubstrateAandB.Thenthesolutionwillturnblueandchangeintoyellowwiththeeffectofacid.TheshadesofsolutionandtheconcentrationofHumanGhrelin(GHRL)arepositivelycorrelated.


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