Bovine Nuclear Factor NF-kappa-B P105 Subunit(NFKB1) ELISA Kit
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Product Name:
Bovine Nuclear factor NF-kappa-B p105 subunit(NFKB1) ELISA kit
Product Type :
ELISA Kit
Code :
CSB-EL015759BO
Size :
96T,5×96T,10×96T
Uniprot NO. :
A7Z034
Abbreviation :
NFKB1
Protein Biological Process 1 :
Transcription/Transcription regulation
Alias :
DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, p105, p50, DNA binding factor KBF1|NF-kappabeta|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA bi
Species :
Bovine
Protein Biological Process 3 :
Transcription
Sample Types :
serum, plasma, tissue homogenates
Detect Range :
18.75 pg/ml-1200 pg/ml
Sensitivity :
4.7 pg/ml
Assay Time :
1-5h
Sample Volume :
50-100ul
Detection Wavelengt :
450 nm
Availability :
5-7 working days
Research Area :
Cell Biology
Target details :
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.
HGNC :
7794
RGD :
70498
MGI :
97312
Precision :
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of bovine NFKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 86 Range % 80-92 1:2 Average % 92 Range % 87-97 1:4 Average % 94 Range % 88-99 1:8 Average % 103 Range % 96-107
Recovery :
The recovery of bovine NFKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-99 EDTA plasma (n=4) 103 98-107
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1200 2.286 2.245 2.266 2.151 600 1.932 1.803 1.868 1.753 300 1.455 1.416 1.436 1.321 150 0.976 0.988 0.982 0.867 75 0.642 0.631 0.637 0.522 37.5 0.424 0.418 0.421 0.306 18.75 0.259 0.255 0.257 0.142 0 0.116 0.114 0.115
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