PCR Cloning & Subcloning
PCR Cloning and Subcloning
PCR
cloning and subcloning
are two main approaches to amplifying DNA sequences. Synbio Technologies is confident in our ability to clone any target gene into any requested site of any vector system on the basis of our, patent pending, Syno®Platform. The combination of the Syno®2.0 tool and the Clone 3.1 technology allows us to insert the requested genetic sequence into any customer specific vector for cloning.
During PCR cloning Synbio Technologies offers sequence optimizations before introducing target gene materials into selected vectors. This sequence optimization allows us to verify the sequence in one hundred percent accurate to the customer’s request before amplification. According to the model clone and sequence from customers, we will design the necessary primers, clone the PCR product of interest into new vector, and finally verify it by Sanger sequencing. This allows us to tailor make a system to supply our customers with the exact specifications necessary to fit their particular research interest.
Subcloning, another popular process of DNA amplification, utilizes the transformation of the template sequences from one vector to another vector with the same restriction enzyme sites. Synbio Technologies is ready to provide our customers with high quality output and cost effective and efficiency benefits through use of subcloning.
Synbio Technologies offers a wide spectrum of genetic technologies. Combining our proprietary Syno®2.0 and Syno®3.0 platform offers a one-stop service ranging from gene synthesis to cloning constructs of interest. Our experienced and exceptional solutions can built any target gene fragments at any site of any require vectors. There is no project out of Synbio Technologies’ ability.
PCR Cloning & Subcloning Advantages
- 100% Sequence Accuracy :We will quickly and accurately synthesize your requested sequence. The generated sequence will then be verified by Sanger sequencing to guarantee 100% sequence accuracy.
- Customizable Subcloning :Guarantee successful clone at any site in any vector system, both commercial and custom, of interest with special amplified primer design.
- Professional Technical Team :Synbio Technologies’ professional technical support offers you proactive communications on your project processing status.
PCR Cloning & Subcloning Services Specifications
Service Type | fragment length | Turnaround Time (Business Day) | Price |
---|---|---|---|
PCR Cloning & Subcloning | <1kb 1-2kb |
10-13 | Quote |
2-3kb | 15-18 | Quote | |
>3kb | Inquiry | Quote |
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Fax +1 609 228 5911 |
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