SPE CARTRIDGES
RayCure SPE series provides high-quality SPE cartridgesfor various types of solid phase extraction, including manifold, vacuum as well as automated solid phase extraction equipment, well compatible with RayKol automated solid-phase extraction systems. RayCure SPE cartridges utilize high-quality sorbents to ensure the efficiency of SPE clean up, offers full range of solid phase extraction cartridgefor various sample matices. Strict quality managment and control ensures high recovery rate of analytes and experiment reproducibility. RayKol provides effective, efficient and economial SPE cartridges with high performance.
SPE CARTRIDGE TYPES
RayCure SPE Column
RayCure SPE series provide pre-packed SPE columns with a variety of sorbents, resins and cartridge size for various methods and target analytes.
RULES TO SELECT RAYCURE SPE CARTRIDGE TYPES
Rule I:
The properties of solid phase packing in SPE cartridges should be match to target compounds.
Rule II:
Take the advantage of differences between analytes of interest and interferences
Rule III:
Consider nature of SPE sample matrices, characteristics of analytes and solubility of used solvents
Users can select RayCure SPE columns depending on properties of desired analytes and sample matrices as following selection guides.
Target compounds |
Properties of analytes |
Corresponding SPE |
||
Solubility |
Polarity/Ionicity |
SPE mechanism |
Sorbent phase |
|
Water soluble |
Compounds with high/low polarity |
Non-polar |
C18 |
|
Polymer |
HLB |
|||
Ionic compounds |
Anion exchange |
MCX, WCX |
||
Cation exchange |
MAX, WAX |
|||
Soluble in organic solvents |
Polar compounds |
Normal phase bonding |
PSA, NH2 |
|
Compounds with neutral polarity |
Normal phase adsorption |
Silica, Florisil |
||
Non-polar compounds |
Reversed phase |
C18 |
Based on nature of SPE sample matrices
SPE sample matrices
SPE sample matrices |
Target compounds |
Impurities |
RayCure SPE column |
Fruits, vegetables, fruit juice, vegetable juice, fruit wine, herbs |
Multiple pesticide residues |
Carbohydrates, pigments, organic acid, phenol |
RayCure QuEChERS d-SPE kits |
RayCure NH2 |
|||
RayCure PSA |
|||
RayCure GCB/NH2 |
|||
RayCure GCB/PSA |
|||
RayCure Florisil |
|||
Weak polar pesticides |
Carbohydrates, pigments, organic acid, phenol |
RayCure HLB |
|
RayCure C18 |
|||
Alkali pesticides |
Carbohydrates, pigments, organic acid, phenol |
RayCure MCX |
|
RayCure WCX |
|||
Acidic pesticides |
Carbohydrates, pigments, organic acid, phenol |
RayCure MAX |
|
RayCure WAX |
|||
Dairy products, blood, urine, animal tissues |
Neutral, weak alkali, weak acidic drugs |
Proteins, fats |
RayCure HLB |
RayCure C18 |
|||
Alkali drugs |
Proteins, fats |
RayCure MCX |
|
RayCure WCX |
|||
Acidic drugs |
Proteins, fats |
RayCure MAX |
|
RayCure WAX |
|||
Soil, wastewater |
hydrophobic organic pollutants |
Humic substances |
RayCure HLB |
RayCure C18 |
|||
Pesticide residues |
Humic substances |
RayCure Florisil |
|
Oil and grease |
Fat-soluble vitamins, phospholipids, aflatoxins |
Fats |
RayCure Silica |
RayCure NH2 |
|||
RayCure PSA |
HOW DOES SPE COLUMN WORK?
SPE is a conventional and efficient extraction technique, it uses the affinity of solutes dissolved or suspended in mobile phase for stationary phase to separate desired compounds from a mixture. And there are two solid phase extraction mechanisms to obtain target analytes from complex samples, to retain desired compounds in stationary phase or retain impurities in solid phase. Depending on the difference of two mechanisms, SPE procedures could be a bit various.
When sample portion passes through SPE column, undesired impurities are discarded with mobile phase, target compounds are retained in stationary phase, then rinse stationary phase with eluents to collect target compounds. It is the most commonly used purification mechanism of solid phase extraction.
In the opposite, to retain impurities in stationary phase, target compounds and few impurities are collected in mobile phase when sample portion passes through SPE column. Rinse the stationary phase which impurities is retained with solvents to obtain rest of retained target compounds. This mechanism is mostly used to eliminate fat-soluble impurities of ion analysis and analysis of multiple pesticides in fruits and vegetables.
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