Human Arginine vasopressin(AVP)ELISA Kit

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Thiskitusesenzyme-linkedimmunesorbentassay(ELISA)basedontheBiotindoubleantibodysandwichtechnologytoassaytheHumanArgininevasopressin(AVP).AddArgininevasopressin(AVP)tothewells,whicharepre-coatedwithArgininevasopressin(AVP)monoclonalantibodyandthenincubate.Afterthat,addantiAVPantibodieslabeledwithbiotintounitewithstreptavidin-HRP,whichformsimmunecomplex.Removeunboundenzymesafterincubationandwashing.AddsubstrateAandB.Thenthesolutionwillturnblueandchangeintoyellowwiththeeffectofacid.TheshadesofsolutionandtheconcentrationofHumanArgininevasopressin(AVP)arepositivelycorrelated.

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