Biopharmaceutical Equipment and Devices
Hanbon is a manufacturer of liquid ssmb chromatographyseries products with a large production scale and a complete range of products in China.
The main products include: Biopharm purification equipment, Oligo synthesizer, industrial preparation simulated moving bed smb chromatographysystems, supercritical fluid chromatography systems, continuous chromatography systems, laboratory chromatography instruments, etc., and can provide users with integrated solutions and services centered on chromatographic products.
Synthesis Principle Of Oligonucleotide
Most oligonucleotides are currently synthesized by solid-phase synthesis, using automated equipment, in which phosphoramidite monomers are required. For example, the phosphoramidite monomer for DNA synthesis mainly consists of bases, deoxyribose, 2-cyanoethyl 5'-DMT and 3'-terminus, and diisopropylamine group, while the phosphoramidite monomer for RNA requires protection of 2'-OH to prevent cross-reaction between 2'-OH and 5'-OH in solid-phase synthesis, and the protecting group is usually TBDMS. Solid-phase synthesis is frequently used nowadays, the traditional solid-phase carriers were controlled microporous glass beads (CPG), but the loading capacity was limited, only 70-80 μmol/g. Nowadays, more high loading capacity solid-phase carriers are used, and the loading capacity is generally up to 250umol/g~350umol/g.
Purification Method Of Oligonucleotide
Oligonucleotides are short-stranded nucleic acids consisting of less than 20 nucleotide units, and purification methods can be selected according to different oligonucleotide lengths, sequences, structures, and purity requirements. The following are some common oligonucleotide purification methods:
Above are some common methods for oligonucleotide purification, Hanbon can offer relevant equipment, which can be used during the RP-HPLC
Choose Hanbon for Your Biopharmaceutical Process
In the synthesis stage, we can provide different sizes of synthesizers to meet your needs in different stages of optimization and next step scale-up, from laboratory level to industrial production level;
In the purification stage, whether you choose reversed-phase chromatography or ion exchange methods, we have the corresponding equipment for you to choose from;
In the ultrafiltration stage, we can also provide different sizes of equipment to meet your needs for salt exchange concentration.
In a word, with Hanbon, you can find the system that matches your need for your production process.
For more informationabout simulated moving bed adsorptionand simulated moving bed chromatography principle, please feel free to contact us!
在线联系供应商
Other supplier products
 |
Preparative Liquid Chromatography System | Hanbon preparative liquid chromatography system has the characteristics of high efficiency, high speed, high automation, and explosion-proof, and i... |
 |
Continuous Chromatographic System | Continuous chromatography systems By implementing this approach, the utilization rate of the ion exchange column can be enhanced, resulting in inc... |
 |
Biopharm Purification Equipment | Biopharm purification equipment independently developed by Hanbon includes Chromatography columns, Chromatography system, In-line buffer dilution s... |
 |
Preparative HPLC | Hanbon offers Hanbon preparative HPLCand MPLC from laboratory to industrial level, which are essential tools for the isolation and purification of ... |
 |
DAC® Dynamic Axial Compression Column | Dynamic axial compression columnare the most mature areas of preparative chromatography using the technology of packing column, dynamic axial compr... |
Same products
 |
Medical Disposable Iv Infusion Set Iv Drip Set | 卖方: Hebei Zongkai Technology Co., LTD | Customization options Customized logo(Min. order: 100000 units) Customize... |
 |
Air Permeability Tester STD- F8 | 卖方: Standard International Group (HK) Limited | The Air Permeability Tester is designed to quickly and accurately measure the breathability and p... |
 |
Surface Performance Tester STD-FB4 - A | 卖方: Standard International Group (HK) Limited | Introduction TheSurface Tester analyzes finger movements-particularly, strokes-performed by arti... |
 |
Compression Tester STD - FB3 - A | 卖方: Standard International Group (HK) Limited | TheCompression Tester analyzes hand movements-particularly, pushing with a finger-performed by ar... |
 |
Single - Strand Bending Tester STD - FB2 - SH | 卖方: Standard International Group (HK) Limited | Standards ASTM A370. ASTM A416. ISO6934.4 The Single Fiber Bending Tester makes it possible to ... |
