Colony Counter

Liquid Chromogenic Culture Medium

Coliforms and E. coli liquid chromogenic medium

Performance Characteristics of Liquid Chromogenic Culture Media

Product Details of Liquid Chromogenic Culture Media

Name	Model	Application	Capacity	Packing	Validity Period
Liquid chromogenic culture medium	XM-30	For 1ml sample inspection	10ml/cup	128 cups/carton	1 year
Liquid chromogenic culture medium	XM-31	For 10ml sample inspection	10ml/cup	128 cups/carton
Liquid chromogenic culture medium	XM-32	For 5ml sample inspection	5ml/cup	128 cups/carton
XM powder medium	XM0001	For preparing medium		20 bags/carton	3 years
MT opener	OS-01	For MT container opening	/	1pc	/

Usage Method of Broth Media Microbiology

3. microbiology sample testdiluent: use a pipette to inject the sample dilution into the cup

XM-30: 1ml, XM-31: 10ml, XM-32: 5ml

※XM powder medium usage: Dissolve 500ml of pure water per bag, inject 10ml into the test tube, autoclave, then inject 1ml sample, and incubate at 36±1°C for 24 hours. The identification method is the same.

Coloring Principle

Coliforms positive

There is a special β-galactosidase only exit in the coliforms. It can be used as an indicator enzyme for coliform bacteria, which catalyzes the hydrolysis of the substrate X-GAL to form 5,5-dibromo -4,4-dichloro indigo, appearing light blue ~ purple-blue.

β--glucuronidase can be used as an indicator enzyme for Escherichia coli; because β-glucuronidase can be observed in Escherichia coli, Salmonella and Shigella in intestinal bacteria, and 95% of E. coli contains β-glucuronidase. It catalyzes the hydrolysis of MUG to form 4-methylumbelliferon, which exhibits fluorescence at 366 nm ultraviolet light and is visually discernible under indoor light intensity.

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