Fmoc / t-Bu Solid Phase Synthesis
The fmoc ltdmethod is a new method for solid phase synthesis of peptides developed by Carpino and Hart based on the Boc method.
The fundamental difference between the Fmoc/t-Bu(9-fluorenylmethoxycarbonyl/tert-butoxy) strategy and the Boc/Bzl strategy is the use of alkali-removable Fmoc is a protecting group for α-amino group, the side chain is protected by t-Bu which can be removed by TFA, and the solid phase carrier Wang resin with acid-sensitive linking arm is used, and the final step of the synthesis is removed by TFA. Han Xiang et al. successfully synthesized the 32-peptide thymosin α 1 by Fm oc solid phase method, starting from Fm oc-Asn(Trt)-W angResin, Boc protecting the side chain amino group of Lys, and tert-butyl ester group (O-t-Bu Protecting the side chain carboxyl group of Asp and Glu, t -Bu protects the side chain hydroxyl group of Ser and Thr, and trityl group (Trt) protects the side chain amide group of Asn, and the total synthesis yield is 33.2%, and the purity is >98.8%.
The Fmoc protected amino acid is sensitive to bases, especially secondary amines, and can be removed with piperidine at room temperature. Under these conditions, Z-based, Boc-based or other amino protecting groups are unaffected.
The Fmoc protecting group is stable to acid and can be removed under mild alkaline conditions. It can be used orthogonally to acid-sensitive side chain protecting groups, and has the advantages of mild reaction conditions, less side reactions, and high yield.
Purification of the product is also facilitated when the polyaryl analog of the Fmoc protecting group is attached to the N-terminus of the polypeptide and protein. At the same time, the Fmoc group has characteristic ultraviolet absorption and is easy to monitor and control the reaction, so it is more and more popular.
Moreover, with the development of the peptide synthesis industry, the cost of Fmoc to protect amino acid raw materials is greatly reduced, and the Fmoc method has gradually replaced the Boc method and is widely used in solid phase synthesis.
Sunresin is an innovation oriented high-tech enterprise. It is specialized in R&D, manufacturing and sales of adsorption and separation polymer resins, equipments and customized solutions including support services. As the first and the only A-share listed company in chinese ion learning exchangeand Adsorption Resin Industry, Sunresin manufactures about 50,000 cubic meters of ion exchange and adsorption resins annually, and supplies customers with tens of adsorption and separation equipment.
Отправить запрос, связаться с поставщиком
Другие товары поставщика
 |
Cane Sugar Refining 21 | Ion Exchange Technique for Sugar Decolorization Resinand Deashing The usual process of cane sugar is extraction, purification, evaporation, concen... |
 |
Wastewater Treatment＆Reuse | Ion Exchange Solutions for Wastewater Treatment system Wastewater treatment is the process of converting wastewater into an effluent that can be d... |
 |
Periodic Table of Elements | Lanthanides The lanthanide series of chemical elements comprises the fifteen metallic chemical elements withatomic numbers 57 through 71, from lant... |
 |
Aminomethyl Resin | Resin Purification Technique Aimed at Antibiotics and Harmful Substances Bee honey is made from the nectar collected by the bees in the hive. Honey... |
 |
Chromatographic Media 21 | SEPLIFE® Chromatography medias Chromatography separation medias are based on Agarose & Dextran& polymer matrix. Chromatography is a t... |
Похожие товары
 |
Hot Melt Adhesive for Vacuum Insulation Panels | Продавец: Foshan Rurga New Material Technology Co. ,Ltd | Hot Melt Adhesive for Vacuum Insulation Panels Supply & Technical Support Capabilities ... |
 |
Hot Melt Adhesive for Packaging | Продавец: Foshan Rurga New Material Technology Co. ,Ltd | Hot Melt Adhesive for Packaging Bond Strength & Durability: Hot melt adhesives provide i... |
 |
Hot Melt Adhesive for Hygiene Products | Продавец: Foshan Rurga New Material Technology Co. ,Ltd | Hot Melt Adhesive for Hygiene Products refers to thermoplastic pressure‑sensitive adhesives ... |
 |
LSAW Steel Pipe & Tube | Продавец: HEBEI TIRICO PIPELINE CO.,LTD | LSAW Steel Pipe & Tube , or Longitudinal Submerged Arc-Welding Pipe (also known as SAWL ... |
 |
Anti-Corrosion Steel Pipe & Tube | Продавец: HEBEI TIRICO PIPELINE CO.,LTD | Anti-Corrosion Steel Pipe & Tube Anti-Corrosion Steel Pipe & Tube What Is an Anti... |
