.

Freeze-drying preservation

  The preservation method is to sublimate the bacterial liquid in a frozen state.
  Finally, a dried sample of the cells was obtained. It also has dry, low temperature and anoxic strain preservation conditions. Therefore, the microbial strain can be preserved for a long period of time, and the freeze-dried strain is sealed in a small ampoule, thereby avoiding contamination during storage. It is also convenient for large-scale preservation. It is currently widely recognized as a method of preservation of strains. However, the operation of the method is relatively cumbersome and technically demanding.
  According to the literature, except for filamentous fungi that produce spores without mycoplasma, it is not suitable for this method. Most other microorganisms, such as viruses, bacteria, actinomycetes, yeasts, filamentous fungi, etc., can be lyophilized and preserved. Many strains can be preserved for more than 10 years using this method.
  The vacuum freeze-drying device has various forms, and the vacuum dryer with a single tube type, a manifold type, a bell type, a centrifugal type, a cabin type, and the like can be selected for the work required for the report. The more the type of the arrangement, the larger the amount of processing and the more complex the device.
  The survival rate of lyophilized strains is affected by many aspects, and different organisms are not able to withstand the process of freezing. Therefore, some strains are not suitable for preservation by lyophilization. The culture conditions and bacterial age before lyophilization are also influencing factors. Cells cultured to stable phase under suitable conditions and mature spores have strong tolerance to lyophilization. Promote the use of more concentrated bacterial suspension. Although its survival rate is low, the absolute amount is relatively high. Protective squeezing has a great impact on survival, and the protective effect is closely related to the chemical structure of the protective agent. Effective protection Liu has a strong affinity for fine saturated water. Skim milk as a protective agent has satisfactory results for a variety of microorganisms. Slow freezing will damage cells, and freezing speed is too fast (freezing in a few seconds) It will form ice crystals in the cells, damage the cell membrane and affect survival. Freeze-dried tubes should be protected from light, especially to avoid direct light. Proper recovery of culture conditions can increase survival.
If you have the needs of freeze dryers, freeze dryers, etc., you need to know the quotation, Please go to to find the or : Contact us, we will contact you in the first time.



Отправить запрос, связаться с поставщиком

Кому: Beijing Songyuan Huaxing Technology Development Co., Ltd.
Ваш E-mail:
Текст письма:


Send to other suppliers

Другие товары поставщика

The most critical compressor selection method for freeze dryers
The most critical compressor selection method for freeze dryers The most critical compressor selection method for freeze dryers The reason why thecan be used is very considerable. In fact, the main reason is al...
LGJ-18 top press type freeze dryer
LGJ-18 top press type freeze dryer The freeze dryer consists of a refrigeration system, a vacuum system, a heating system, and an electrical instrument control system. The main compo...
Freeze-dried powder freeze dryer
Freeze-dried powder freeze dryer In the beauty and cosmetics market, the active cosmetics freeze-dried powder and freeze-dried mask of the net red are currently popular! Do you kno...
LGJ-20F Gland Type Experimental Freeze Dryer
LGJ-20F Gland Type Experimental Freeze Dryer Freezing and drying process of LGJ-20F (silicon oil heating) are finished in the same place (patented product). It changes the cumbersome operation...
LGJ-12S Multi Manifold Top Press Type Experimental Freeze Dryer
LGJ-12S Multi Manifold Top Press Type Experimental Freeze Dryer  Technical parameters:   freeze drying area:0.08㎡   tray dia.:Ф180mm   tray numbers:3pcs   tray spacing:70mm   flask capacity & q'ty:1...
Все товары поставщика

Похожие товары

The latest fashion design of 2020 is unbreakable, TSA locks the trunk-PPZ1801
The latest fashion design of 2020 is unbreakable, TSA locks the trunk-PPZ1801 Продавец: Jiaxing Tianhao Industry Location: Zhejiang, China Business Type: Manufacturer, Exporter Brands: Ti...
Titanium Blocks
Titanium Blocks Продавец: Ningbo Chuangrun New Materials Location: China Business Type: Manufacturer, Exporter Brands: CRNMC C...
Потомственный Сибирский колдун. Приворот. Гадания.
Потомственный Сибирский колдун. Приворот. Гадания. Продавец: Магическая помощь в любых ситуациях! Я потомственный Сибирский маг в пятом поколении. Работаю и помогаю людям с 1989 года. Я оказываю ...
THE PROCESS OF VENEER SEWING MACHINE
THE PROCESS OF VENEER SEWING MACHINE Продавец: 742462 Theis suitable for core joining together in plywood production line. It can work different length...
Note for Planer and sander machine
Note for Planer and sander machine Продавец: 742462 Speaking ofPlaner and sander machine, many friends may not know. I have heard of sanders and poli...