.

Synbio Technologies PCR cloning and Subcloning Technology

PCR cloning andsubcloningtechnology, first developed in the 1970s, is now a staple in every molecular biology lab in the world. Cloning allows researchers to much more easily understand gene function at a deeper level, and greatly facilitates gene editing. PCR cloning and subcloning technology is not only revolutionary for the field of biology, but has profound implications on fields like agriculture, industry, and medicine as well.

Subcloningvs molecular cloning

PCR cloning technology

PCR cloning technology is similar to natural DNA replication, and contains three basic reaction steps: modification-annealing-extension. We can obtain a desired target gene sequence with appropriate primer design. PCR can then be used to amplify this gene sequence, preparing it for use in cloning.

Subcloning technology

In molecular cloning, target DNA is assembled into a vector plasmid through restriction enzymes and screening. In subcloning, a gene of interest is transferred from one vector to another. Both processes consist of several key steps, such as screening of the target fragment, cloning vector preparation, transformation/transduction of the product into cells, and screening for cells containing recombinant plasmids.

Both PCR cloning and subcloning technology can insert a target gene into a plasmid of choice in vitro through recombinant technology. The main forms of target gene transfer into a plasmid are transformation and transduction. This allows researchers to have an enormous amount of customization available to them when trying to study a gene of interest, making cloning and sub-cloning two extremely powerful tools in a molecular biologist’s arsenal.

With our proprietary Syno®2.0 gene synthesis platform, Synbio Technologies can provide one-step services for gene synthesis, vector construction, PCR cloning, and subcloning. Customers only need to offer the sequence information, and we can help design amplification primers and clone the PCR products to the specific sites of the new plasmid. We also provide sequencing services in order to confirm that the correct product was accurately synthesized.



Отправить запрос, связаться с поставщиком

Кому: synbio технологий
Ваш E-mail:
Текст письма:


Send to other suppliers

Другие товары поставщика

Syno® 2.0 Gene Synthesis Services
Syno® 2.0 Gene Synthesis Services Gene Synthesis Synbio Technologies is one of the leading companies insynthetic biologyindustry, especially with respect to gene synthesis . We h...
Codon Optimization
Codon Optimization Codon optimization Codon optimization refers to the use of preferred codons, that is, to avoid the rare codons with low utilization, to simplify m...
PCR Cloning & Subcloning
PCR Cloning & Subcloning PCR Cloning and Subcloning PCR cloning and subcloning are two main approaches to amplifying DNA sequences. Synbio Technologies is confident in o...
Syno® 2.0 Gene Synthesis Services
Syno® 2.0 Gene Synthesis Services Gene Synthesis Synbio Technologies is one of the leading companies insynthetic biologyindustry, especially with respect to gene synthesis. We have...
Protein Expression and Purification
Protein Expression and Purification Still struggling with DNA sequence optimization and downstream expression? Still busy troubleshooting to protein purification?Is your project stall...
Все товары поставщика

Похожие товары

pulse oximeter
pulse oximeter Продавец: Shanghai Berry Electronic Tech Co., Ltd. Feature Convenience Compact appearance, more convenient to carry.A button to wake up, even the e...
100mL Personal Lubricant Warming Based
100mL Personal Lubricant Warming Based Продавец: Health & Beyond Inc. 100mL Personal Lubricant Warming Based Volume:100ml Our warming-based formula is enriched with ...
Synbio Technologies PCR cloning and Subcloning Technology
Synbio Technologies PCR cloning and Subcloning Technology Продавец: synbio технологий PCR cloning andsubcloningtechnology, first developed in the 1970s, is now a staple in every mol...
Synthetic Biology Definition
Synthetic Biology Definition Продавец: synbio технологий Synthetic biologydefinitionrefers to the design and construction of novel biological components, ...
Protein Directed Evolution
Protein Directed Evolution Продавец: synbio технологий Protein directed evolution Library In vitrodirected evolution of proteins is an artificially s...