BlueGene Biotech Guinea Pig Eosinophil Cationic Protein ELISA kit
E05E0125 Guinea Pig Eosinophil Cationic Protein ELISAkit
Guinea Pig Eosinophil cationic protein ELISA kit is suitable for the detection of samples from guinea Pig species. Eosinophil cationic protein can also be called ribonuclease 3, Rnase-A3, RNASE3, ECP, RNS3, RAF1, ribonuclease A family member 3.
Principle of the Assay
ECP ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-ECP antibody and an ECP-HRP conjugate. The assay sample and buffer are incubated together with ECP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ECP concentration since ECP from samples and ECP-HRP conjugate compete for the anti-ECP antibody binding site. Since the number of sites is limited, as more sites are occupied by ECP from the sample, fewer sites are left to bind ECP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECP concentration in each sample is interpolated from this standard curve.
As a professional drug residue test kit factory, Bluegene elisahas high-quality drug residue test kitfor sale. Welcome to contact us if needed.
Send product request
Other supplier products
 |
Human ELISA Kits | Our more than 8000 human visfatin elisa kitare intended to advance your research to study human disease covering several research areas (like cance... |
 |
Human Adenosine Triphosphate ELISA kit | E01A0038 Human Adenosine Triphosphate ELISA kit Human Adenosine Triphosphate ATP ELISAkit is suitable for the detection of samples from human sp... |
 |
BlueGene Biotech Canine β Cell Leukemia/Lymphoma 2 ELISA kit | E08B0033 Canine β Cell Leukemia/Lymphoma 2 ELISA kit Canine β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samp... |
 |
Human/Canine/Porcine Insulin ELISA kit | NE01I0004 Human/Canine/Porcine elisa insulin kit Human/Canine/Porcine elisa for insulinkit is suitable for the detection of samples from human s... |
 |
BlueGene Biotech Bovine Decorin ELISA kit | E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of samples from Bovine species. Decorin can also be call... |
Same products
 |
Peptide Technology Cooperation & Transfer | Seller: PeptiOrigin Biotechnology Co., Ltd. | Peptide Technology Cooperation & Transfer Driving Innovation Through Strategic Partnerships ... |
 |
Recombinant Human Fibroblast Growth Factor-basic | Seller: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd | Physical Appearance:Freeze-dried powder Formulation:5mMPB(pH7.4) before freeze-drying TheorypI:... |
 |
Recombinant Staphylococcal Protein A | Seller: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd | TheorypI:5.10 Resources:Escherichia coli(E. coli) Purity:≥95% Storage condition:-20℃ Stora... |
 |
NuPerley Core 700 | Seller: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd | NuPerley Core 700 Matrix：Rigid agarose microsphere Ligand：n-octylamine Particle size, d50：~90 ... |
 |
Q NUPharose Fast Flow | Seller: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd | Matrix：6% Cross-linked agarose Ligand：Quaternary ammonium salt, -CH2CH(OH)CH2N(CH3)3+ Particle ... |
