BlueGene Biotech Porcine Deoxyribonuclease I ELISA kit
E07D0214 Porcine Deoxyribonuclease I ELISA kit
The Porcine Deoxyribonuclease I ELISA kit is suitable for the detection of samples from porcine species. Deoxyribonuclease I can also be called DNaseI, DNL1, DRNI, DNase-I, Dornase alfa.
Principle of the Assay
Dnase I ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-Dnase I antibody and an Dnase I-HRP conjugate. The assay sample and buffer are incubated together with Dnase I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Dnase I concentration since Dnase I from samples and Dnase I-HRP conjugate compete for the anti-Dnase I antibody binding site. Since the number of sites is limited, as more sites are occupied by Dnase I from the sample, fewer sites are left to bind Dnase I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Dnase I concentration in each sample is interpolated from this standard curve.
As a professional drug residue test kit factory, Shanghai bluegene biotechhas high-quality human insulin elisa kitfor sale. Welcome to contact us if needed.
Send product request
Other supplier products
 |
Rat Epinephrine/Adrenaline ELISA kit | E02A0007 Rat Epinephrine/Adrenaline ELISA kit Rat Adrenaline/Epinephrine ELISA kitis suitable for the detection of samples from Rat species. Epi... |
 |
ELISA Kits | The elisa kit companyShanghai BlueGene Biotechhas focused on the research and development of ELISA kits, especially for more than a decade. Our ... |
 |
BlueGene Biotech Canine Caspase 9 ELISA kit | E08C0096 Canine Caspase 9 ELISA kit Canine Caspase 9 ELISA kitis suitable for the detection of samples from Canine species. Caspase 9 can also b... |
 |
BlueGene Biotech Canine Endothelin 1 ELISA kit | E08E0040 Canine Endothelin 1 ELISAkit Canine Endothelin 1 ELISA kitis suitable for the detection of samples from Canine species. Endothelin 1 ca... |
 |
BlueGene Biotech Mouse Angiotensin 2 ELISA kit | E03A0204 Mouse Ang2 ELISAkit Mouse Angiotensin 2 ELISA kitis suitable for the detection of samples from mouse species. Angiotensin 2 can also be... |
Same products
 |
Peptide Technology Cooperation & Transfer | Seller: PeptiOrigin Biotechnology Co., Ltd. | Peptide Technology Cooperation & Transfer Driving Innovation Through Strategic Partnerships ... |
 |
Recombinant Human Fibroblast Growth Factor-basic | Seller: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd | Physical Appearance:Freeze-dried powder Formulation:5mMPB(pH7.4) before freeze-drying TheorypI:... |
 |
Recombinant Staphylococcal Protein A | Seller: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd | TheorypI:5.10 Resources:Escherichia coli(E. coli) Purity:≥95% Storage condition:-20℃ Stora... |
 |
NuPerley Core 700 | Seller: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd | NuPerley Core 700 Matrix：Rigid agarose microsphere Ligand：n-octylamine Particle size, d50：~90 ... |
 |
Q NUPharose Fast Flow | Seller: Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd | Matrix：6% Cross-linked agarose Ligand：Quaternary ammonium salt, -CH2CH(OH)CH2N(CH3)3+ Particle ... |
