BlueGene Biotech Porcine Deoxyribonuclease I ELISA kit
E07D0214 Porcine Deoxyribonuclease I ELISA kit
The Porcine Deoxyribonuclease I ELISA kit is suitable for the detection of samples from porcine species. Deoxyribonuclease I can also be called DNaseI, DNL1, DRNI, DNase-I, Dornase alfa.
Principle of the Assay
Dnase I ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-Dnase I antibody and an Dnase I-HRP conjugate. The assay sample and buffer are incubated together with Dnase I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Dnase I concentration since Dnase I from samples and Dnase I-HRP conjugate compete for the anti-Dnase I antibody binding site. Since the number of sites is limited, as more sites are occupied by Dnase I from the sample, fewer sites are left to bind Dnase I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Dnase I concentration in each sample is interpolated from this standard curve.
As a professional drug residue test kit factory, Shanghai bluegene biotechhas high-quality human insulin elisa kitfor sale. Welcome to contact us if needed.
在线联系供应商
Other supplier products
 |
Growth Factor Proteins | Growth factors are a kind of soluble signal molecules, which are necessary to regulate the normal growth and metabolism of microorganisms, but they... |
 |
Human Chemokine (C-C motif) Ligand 18 ELISA kit | Immunology NE01C0064 Human Chemokine (C-C motif) Ligand 18 ELISA Kit Human Chemokine (C-C motif) ligand ccl18 elisakit is suitable for the dete... |
 |
BlueGene Biotech Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit | E07C0209 Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit Porcine soluble Cluster of differentiation 40 ligand ELISA kit is suitab... |
 |
Human Collagen, Type I, Alpha 1 ELISA kit | NE01C1899 Human Collagen, Type I, collagen 1 elisakit Human Collagen, type I, collagen type i alpha 1kit is suitable for the detection of sample... |
 |
Fish ELISA Kits | Fish ELISA Kits Fish are often used in environmental monitoring, embryo research, tumor research and others. Among them, zebrafish is the most com... |
Same products
 |
Chemical Products for Industrial Applications | 卖方: 华诺电气有限公司 | Our chemical products are developed for a wide range of industrial applications, including manu... |
 |
NPK 13-5-40+TE Water-soluble Fertilizer | 卖方: Hebei Lvwang Ecological Fertilizer Co., Ltd. | NPK 13-5-40+TE Water-soluble Fertilizer for Vegetables and Fruits NPK 13-5-40+TE water-soluble f... |
 |
China Fertilizer Factory NPK 12-24-12 Compound Fertilizer for Rice | 卖方: Hebei Lvwang Ecological Fertilizer Co., Ltd. | China Fertilizer Factory NPK 12-24-12 Compound Fertilizer for Rice NPK 12-24-12 compound Fertili... |
 |
2-Amino-6-chloropurine 10310-21-1 | 卖方: Xinxiang Ruicheng Technology Co.,Ltd. | 2-Amino-6-chloropurine 10310-21-1 2-Amino-6-chloropurineis a reactive purine compound used as a ... |
 |
Xanthine 69-89-6 | 卖方: Xinxiang Ruicheng Technology Co.,Ltd. | Xanthine 69-89-6 Xanthineis a natural purine base that serves as a key intermediate in purine me... |
