Melamine ELISA Test Kit

Melamine ELISA Test Kit

Catalog No. LSY-10026

This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained.

Sensitivity: 1 ppb

Incubator temperature: 25℃

Incubator time: 30min～15min

Detection limit:

Milk powder................................................................................... 20 ppb

Milk............................................................................................... 16 ppb

Milk/Milk powder (Method two).......................................................... 1 ppb

Tissue (chicken, pork, duck, fish, shrimp and liver)............................. 2 ppb

Feed............................................................................................ 100 ppb

Egg............................................................................................... 20 ppb

Serum............................................................................................. 4 ppb

Cross-reaction rate:

Melamine......................................................................................... 100%

Cyanuric acid..................................................................................... 60%

Triazine.............................................................................................. <1%

Diamino atrazine................................................................................ <1%

Recovery rate:

Milk/Milk powder........................................................................... 90±19%

Tissue.......................................................................................... 85±20%

Feed............................................................................................ 85±20%

Egg.............................................................................................. 80±20%

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each): 0 ppb,1 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb

3） Enzyme conjugate (7 mL)............................................................... red cap

4） Antibody working solution (7 mL).................................................. blue cap

5） Substrate A solution (7 mL).......................................................... white cap

6） Substrate B solution (7 mL)......................................................... black cap

7） Stop solution (7 mL).................................................................. yellow cap

8） 20× concentrated washing buffer (40 mL)..................................... white cap

9） 2× concentrated redissolving solution (50 mL)..................... transparent cap

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