Chromatography System

Hanbon advanced chromatography systemshas reliable performance and flexible configuration, and can provide Bio-Lab® laboratory chromatography system, Bio-Pro® pilot&process chromatography system, etc.

ommon Principles Of Process Scale-Up: Linear Scale-Up

Linear scale-up is the most common principle used in process scale-up. By linear scale-up, we mean that we can obtain almost uniform bioprocess chromatographyresults from laboratory scale to pilot results or industrial production level. The core idea is to keep the column height and linear flow rate constant and only scale up the column diameter and volume flow rate. A fixed column height and linear flow rate theoretically ensures the same retention time, the number of CVs used in chromatography and the number of CVs of the eluted sample, maximizing the same purification results. Theoretically, this linear scale-up of the purification separation behavior should be very similar, thus ensuring a successful scale-up of the ion chromatography process. However, some additional factors need to be considered, including a decrease in column tube support (increased pressure drop), column efficiency/column bed instability, sample diffusion (peak spreading), and dead volume of the system and tubing.

Conversion Of Linear Flow Rates (Cm/H) To Volumetric (Ml/Min) Flow Rates

When comparing results from columns of different sizes, it is convenient to express the flow rate as a linear flow rate (cm/h). However, flow rates are often measured as volumetric flow rates (ml/min). In order to convert between linear and volumetric flow rates, one of the following equations can be used .

Linear flow rate (cm/h) to volumetric (ml/min) flow rate

Volumetric flow rate (ml/min) = linear flow rate (cm/h) / 60 x column cross-sectional area (cm2)

=Y/60×π×d2/4

Y = linear flow rate (cm/h)

d = internal diameter of the column (cm)

There is a glass column (inner diameter 1.6 cm), when the linear flow rate is 150 cm/h, what is the volumetric flow rate?

Y=Linear flow rate = 150cm/h

d = inner diameter of the column = 1.6 cm

Volumetric flow rate = 150 x π x 1.6 x 1.6/(60 x 4)

From volumetric flow rate (ml/min) to linear flow rate (cm/h)

Linear flow rate (cm/h) = volumetric flow rate (ml/min) x 60/column cross-sectional area (cm2)

=Z×60×4/π×d2

Z = volumetric flow rate of the column (ml/min)

d = inner diameter of the column (cm)

There is a glass column (inner diameter 0.5 cm), when the volumetric flow rate is 1 ml/min, what is the linear flow rate?

Z = volumetric flow rate of the column = 1 ml/min

d = inner diameter of the column = 0.5 cm

Linear flow rate = 1 x 60 x 4/(π x 0.5 x 0.5) cm/h = 305.6 cm/h

We provide chromatography step by step method, chromatography purification method, chromatography process example, anion exchange chromatography elution, equipment used for chromatography, etc. For more information, please feel free to contact us!

Other supplier products

	Chromatographic Column	Hanbon medium and high pressure pilot scale chromatography columninclude DAC（dynamic axial compression）columns and laboratory columns. In 2005, Han...
	Chromatography Column	Hanbon process chromatography columnscan meet the use requirements of most chromatographic fillers. Its good filling effect and high column efficie...
	Depth Filtration System	single use depth filtrationsystems are designed for biopharmaceutical downstream separation and purification. The constant pressure difference, con...
	Buffer Dilution/ Conditioning System	Bio-Con® auto dilution systemis to mix various concentrated buffer solutions, including acid, alkali, salt, etc., with injection water online t...
	Intepure™ Integrated Liquid Chromatograph	Intepure is a flexible and intuitive chromatography system for the rapid purification of milligram to gram scale chemical synthesis, natural produc...

供应产品

Same products

	Discover Bioprocessing Products That Boost Your Biopharmaceutical Process	卖方: 百林科 Bio-Link	Bio-Link provides a range of major bioprocess productto biomanufacturers around the world, includ...
	Soybean oil	卖方: 跨农业	Предлагаем на экспорт из России нерафинированное соевое масло. Налив в флекситанк. Specification:...
	Soybean oil	卖方: 跨农业	Предлагаем на экспорт из России нерафинированное соевое масло. Налив в флекситанк. Specification:...
	Soybean oil	卖方: 跨农业	Предлагаем на экспорт из России нерафинированное соевое масло. Налив в флекситанк. Specification:...
	Soybean oil	卖方: 跨农业	Предлагаем на экспорт из России нерафинированное соевое масло. Налив в флекситанк. Specification:...