Regulatory Standards and Dosage Limits of Nisin

Global regulatory agencies have established strict usage standards for Nisin:
FAO/WHO: The acceptable daily intake (ADI) is set at 0–3300 IU/kg body weight (equivalent to 0–13 mg/kg body weight), based on comprehensive toxicological data.
China (GB 2760): Permitted for use in over 20 food categories, including dairy products, meat products, condiments, and beverages. Maximum addition levels range from 0.05 to 0.5 g/kg (calculated as pure Nisin), with specific limits adjusted by food type (e.g., 0.2 g/kg in soy sauce, 0.15 g/kg in vinegar, 0.5 g/kg in canned meat).
EU (EC No. 1333/2008): Classified as a food additive (E234), with similar dosage limits to China and mandatory labeling requirements for food products containing Nisin.

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