H3K9ME2
Synthesized histone H3 peptide corresponding to residues within 1-135 of human histone H3 with dimethylation K9 modification.
SPECIFICATION OF H3K9ME2
CAT |
H3105 |
Product Name |
Histone H3-K9me2 |
Purity |
≥95% by HPLC analysis |
Appearance |
White powder |
Usage |
Research use only |
Storage |
Shipping at room temperature. Upon delivery aliquot. Stored at -20°C or -80°C. Avoid freeze/thaw cycle. |
Sequence |
ARTKQTARK(me2)S TGGKAPRKQL ATKAARKSAP ATGGVKKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSSAVM ALQEACEAYL VGLFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA |
APPLICATION OF H3K9ME2
H3K9me2is strongly associated with transcriptional repression. H3K9me2 levels are higher at silent compared to active genes in a 10kb region surrounding the transcriptional start site. H3K9me2 represses gene expression both passively, by prohibiting acetylation and therefore binding of RNA polymerase or its regulatory factors, and actively, by recruiting transcriptional repressors. H3K9me2 has also been found in megabase blocks, termed Large Organised Chromatin K9 domains (LOCKS), which are primarily located within gene-sparse regions but also encompass genic and intergenic intervals. Its synthesis is catalyzed by G9a, G9a-like protein, and PRDM2. H3K9me2 can be removed by a wide range of histone lysine demethylases (KDMs) including KDM1, KDM3, KDM4, and KDM7 family members. H3K9me2 is important for various biological processes including cell lineage commitment, the reprogramming of somatic cells to induced pluripotent stem cells, regulation of the inflammatory response, and addiction to drug use.
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