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Human apelin (AP) ELISA Kit

Drug Names
Generic Name:Human apelin (AP) ELISA Kit.
Purpose
This kit allows for the determination of AP concentrations in human serum, blood plasma,
and other biological fluids.
Principle of the assay
The kit assay human AP level in the sample,use Purified human AP antibody to coat
microtiter plate wells, make solid-phase antibody, then add AP to wells, Combined AP which
With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing
Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of AP in the samples is then determined by comparing the O.D. of the samples to
the standard curve.



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Human apelin (AP) ELISA Kit
Human apelin (AP) ELISA Kit Drug Names Generic Name:Human apelin (AP) ELISA Kit. Purpose This kit allows for the determination of AP concentrations in human serum, blood plasm...
Human apelin (AP) ELISA Kit
Human apelin (AP) ELISA Kit Drug Names Generic Name:Human apelin (AP) ELISA Kit. Purpose This kit allows for the determination of AP concentrations in human serum, blood plasm...
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