Synbio Technologies PCR cloning and Subcloning Technology

• 

PCR cloning andsubcloningtechnology, first developed in the 1970s, is now a staple in every molecular biology lab in the world. Cloning allows researchers to much more easily understand gene function at a deeper level, and greatly facilitates gene editing. PCR cloning and subcloning technology is not only revolutionary for the field of biology, but has profound implications on fields like agriculture, industry, and medicine as well.

Subcloningvs molecular cloning

PCR cloning technology

PCR cloning technology is similar to natural DNA replication, and contains three basic reaction steps: modification-annealing-extension. We can obtain a desired target gene sequence with appropriate primer design. PCR can then be used to amplify this gene sequence, preparing it for use in cloning.

Subcloning technology

In molecular cloning, target DNA is assembled into a vector plasmid through restriction enzymes and screening. In subcloning, a gene of interest is transferred from one vector to another. Both processes consist of several key steps, such as screening of the target fragment, cloning vector preparation, transformation/transduction of the product into cells, and screening for cells containing recombinant plasmids.

Both PCR cloning and subcloning technology can insert a target gene into a plasmid of choice in vitro through recombinant technology. The main forms of target gene transfer into a plasmid are transformation and transduction. This allows researchers to have an enormous amount of customization available to them when trying to study a gene of interest, making cloning and sub-cloning two extremely powerful tools in a molecular biologist’s arsenal.

With our proprietary Syno®2.0 gene synthesis platform, Synbio Technologies can provide one-step services for gene synthesis, vector construction, PCR cloning, and subcloning. Customers only need to offer the sequence information, and we can help design amplification primers and clone the PCR products to the specific sites of the new plasmid. We also provide sequencing services in order to confirm that the correct product was accurately synthesized.

Другие товары поставщика

	Humanized Antibody Library	Humanized antibody (library) service Traditional humanized antibody library mainly refers to a group of re-expressed antibody which is transformed...
	Molecular Biology Services	Molecular biology services Molecular biology is the core of many life science research topics, and also plays a fundamental role in modern medical...
	Syno® 2.0 Gene Synthesis Services	Gene Synthesis Synbio Technologies is one of the leading companies insynthetic biologyindustry, especially with respect to gene synthesis . We h...
	Protein Expression and Purification	Still struggling with DNA sequence optimization and downstream expression? Still busy troubleshooting to protein purification？Is your project stall...
	PCR Cloning & Subcloning	PCR Cloning and Subcloning PCR cloning and subcloning are two main approaches to amplifying DNA sequences. Synbio Technologies is confident in o...

Похожие товары

	A-01 Stainless Steel Is Suitable For Hospital Bed Guardrail	Продавец: Hengshui Defeng Medical Equipment Co.,Ltd	A-01 Stainless Steel Is Suitable For Hospital Bed Guardrail The A-01 Hospital Bed Guardrailis ma...
	Walnut Protein Manufacturer	Продавец: Beijing SEMNL Biotechnology Co., Ltd.	Walnut Protein Manufacturer Walnut Peptide is a small molecule collagen peptide extracted f...
	Soy Peptide Powder Manufacturer	Продавец: Beijing SEMNL Biotechnology Co., Ltd.	Soy Peptide Powder Manufacturer Soy Peptide Powder SEMNL is made of soy protein or soy meal...
	ginseng extract manufacturer	Продавец: Beijing SEMNL Biotechnology Co., Ltd.	ginseng extract manufacturer Ginseng Extract , due to its unique ginsenosides, is believed ...
	Enhancing the Patient Experience in Geriatric Care with NeuViz 128	Продавец: 00000	Enhancing the Patient Experience in Geriatric Care with NeuViz 128 SERVIMOVIL, a diagnostic ...