HEPPSO BUFFER CAS NO.68399-78-0
HEPPSO BUFFER CAS NO.68399 78 0
Heppso buffer can form free radicals, so it is not suitable for redox reaction. Heppso forms a complex with Cu (II) ions, so the stability constant and concentration should be considered when using this buffer in solutions containing copper ions. Although heppso is combined with copper ion, it is still suitable for the determination of dioctenic acid (BCA). Protein induced color reduction can be diluted or eliminated by precipitation of protein before reaction with dioctenic acid.
Heppso Buffer Product Description
Heppso buffer is widely used in biochemical experiments and belongs to good's series buffer. It is a zwitterionic piperazine buffer with an effective pH in the range of 7.1 – 8.5. It is usually used as an amphoteric separator to produce a pH gradient in isoelectric focusing. Heppso is not toxic to crustaceans that have been studied and is considered suitable for toxicological studies.
Detailed Introduction of Heppso Buffer
The full name of heppso is 3 - (hydroxyethyl piperazine) - 2-hydroxypropanesulfonic acid, CAS No. 68399-78-0, and the appearance is white powder. The working pH range of heppso buffer is 7.1-8.5, and the pKa value at the concentration of 2.0 mm buffer is 7.84, which has high solubility in water. As a zwitterionic buffer, it has a wide range of applications.
Product Advantages of Heppso Buffer
Heppso powder has good water solubility and simple configuration;
High purity and high chemical stability;
Non toxic to biological cells;
Pure white powder with high clarity after dissolution.
Advantages of Desheng Heppso Buffer
Have a professional R & D and production team, and strictly control the indicators and parameters of heppso;
The finished product has high purity and good use effect;
Products in powder form are easier to transport and preserve than solutions;
High daily output, can provide a large number of spot goods, and the delivery cycle is short;
The manufacturer sells directly, the price is more favorable, and the quantity is more discount.
Application of Heppso Buffer
Heppso buffer is usually used as an amphoteric separator to produce a pH gradient in isoelectric focusing;
Heppso buffer is not toxic to crustaceans that have been studied and is considered suitable for toxicological studies;
Heppso buffer can form free radicals, so it is not suitable for redox reaction;
Heppso buffer forms a complex with Cu (II) ions, so the stability constant and concentration should be considered when using this buffer in solutions containing copper ions;
Although heppso buffer is combined with copper ion, it is still suitable for the determination of dioctenic acid (BCA);
Protein induced color reduction can be diluted or eliminated by precipitation of protein before reaction with dioctylcolic acid.
Preparation Method of Heppso Buffer
Dissolve 268.33 g of heppso powder in 750 mL of dh2o;
Adjust to the required pH value with 10N NaOH;
Fill with dh2o to the final volume of 1 L;
Filtration disinfection (recommended) or autoclave;
Store below 4 ℃.
Heppso Buffer Precautions
Heppso powder needs to be sealed and stored. After preparing the solution, it should be prepared and used immediately to avoid long-term contact with air and affecting the use effect;
When preparing solvent, clean the container more than 3 times to avoid exchange with other substances;
Take the quantity strictly according to the experimental requirements, otherwise the experimental effect will be affected;
If you accidentally touch the skin, please wash it with clean water immediately. If it is serious, you need to see a doctor as soon as possible.
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