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Keratinase

Keratinase Products

Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase enzymenot only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.

Specification of Keratinase

Name

Keratinase

Product Series Name

NutriGen Keratinase

Activity

U/g

Strain

Parengyodontium album

EC code

EC 3.4.99.11

Form

Powder,Liquid

Carrier

Starch

Color

White, Light yellow

Loss on drying

≤ 10%

Working pH

Optimum pH

11

Working T

30℃-70℃

Optimum T

60℃

Particle size

80% pass 40 mesh

Package

25kg /bag

Storage

Stored 6 months in dark, cool and dry conditions

Caution

Avoid direct sunlight, high temperature

Definition and Dosage of Activity Keratinase

One unit (U) of protease activity is defined as the amount of enzyme chinawhich liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.

Recommended dosage is 50-100g/t with 50000 U/g in complete feed.

Functions of Keratinase

Application of Keratinase

Nutrient

Poultry

Value

Amount provided in the diet

Metabolizable energy(Mcal/kg)

600

Crude protein, %

5400

Lysine, %

150

Methionine, %

180

Cystine, %

150

Threonine, %

210

Tryptophane, %

90

Isoleucine , %

150

Arginine, %

285

Valine, %

255

Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.

Experimental design:

Item

Diet

PC

Basal diet

NC

Basal diet-0.5% crude protein

Group I

NC+125g/t competitor

Group II

NC+100g/t Keratinase(30000U/g)

Experimental cycle: 42 days

Results:

Table2 Effect of each treatment on the growth performance of AA broilers (g, %)

Items

PC

NC

Group I

Group II

Initial weight,g

1-21days

21stAG,g

ADFI,g

ADG,g

F/G

Death rate,%

22-42days

22ndAG,g

42ndAG,g

ADFI,g

ADG,g

F/G

Death rate,%

1-42days

ADFI,g

ADG,g

F/G

Death rate,%

Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).

Diagram1 Effect of keratinase on the nutrient digestibility of broilers

Table3 Effect of keratinase on the amino acid digestibility of broilers (%)

Items

PC

NC

Group I

Group II

Asp

Thr

Ser

Glu

Gly

Ala

Val

Met

Ile

Leu

Tyr

Phe

His

Lys

Arg

Pro

TAA

Conclusion of Keratinase

On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinase improve the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, keratinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that keratinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.



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