Antibody Library Technology Screen Out the Specific Antibody

Humanized antibodies are derived from non-human species, modified to more closely resemble existing, natural human antibodies. Their applications have a great deal of potential in medicine and immunology due to the wide range of variability that humanized antibodies provide. Compared with heterologous antibodies, they also circumvent certain immune side effects to the human body. From chimeric antibodies to CDR-grafting to humanized antibodies, each major breakthrough has helped expand the field of antibody preparation and advance the cutting edge ofantibody library technology .

The development of therapeutic antibodies has been tending toward the use of fully humanized antibodies, which are typically derived from antibody libraries. The development of antibody library technology makes it possible to more easily obtain antibodies in vitro. Antibody fragments can be immobilized on the surface of certain mediums, and specific antibody fragments can be obtained by multiple rounds of exposure to a certain antigen, followed by elution and signal amplification. Due to the competitive binding screening mechanism, antibodies with a high-copy number will be preferentially screened out from the library. After 1-2 rounds of screening, high-affinity and low-copy antibodies can then be screened by antibody library sequencing.

The classical antibody library displays phage coat protein III and VIII protein fusion scFv (single-chain variable fragment) on the surface of phage. Fully humanized scFv can be screened out by several rounds of antigen screening. There is now an improved way to screen for scFv completely in vitro, starting from the scFv antibody library. No terminator is inserted during in vitro transcription, following which a RNA-ribosome-scFv complex is formed. The complex which specifically combines to the target molecule can be obtained by a screening method similar to phage display. Finally, the RNA of the complex is separated and PCR amplification follows. The introduction of mutations along with the PCR amplification could stimulate the affinity maturation process and could be one option to amplify the affinity of the secondary antibody library.

Synbio Technologies provides antibody library sequencing services. Combining with our next generation sequencing technology, we offer a complete assessment of immune system diversity and comprehensive observation and analysis of T cells and B cells. These technologies and services. Our services are widely applicable to topics such as disease surveillance, antibody production, vaccine research, medicine, and immunology.

Send product request

To: Synbio Technologies
Your E-mail:
Message text:

Send to other suppliers

Other supplier products

Molecular Biology Services
Molecular Biology Services Molecular biology services Molecular biology is the core of many life science research topics, and also plays a fundamental role in modern medical...
Humanized Antibody Library
Humanized Antibody Library Humanized antibody (library) service Traditional humanized antibody library mainly refers to a group of re-expressed antibody which is transformed...
Protein Expression and Purification
Protein Expression and Purification Still struggling with DNA sequence optimization and downstream expression? Still busy troubleshooting to protein purification?Is your project stall...
Syno® 2.0-Gene Synthesis
Syno® 2.0-Gene Synthesis Syno® 2.0: PCR based enzyme synthesis Syno® 2.0 platform of Synbio Tech. is an industrialized de novo DNA synthesis platform. In Syno®...
CRISPR Cas9 sgRNA Design Center
CRISPR Cas9 sgRNA Design Center CRISPR Cas9 sgRNA Design Center TheCRISPR-Cas9system is a prokaryotic immune system driven by RNA that triggers resistance to foreign genetic elem...
All supplier products

Same products

buy crystal meth
buy crystal meth Seller: 727267 Blacknetsales | buy crystal methamphetamine online blacknetsales.com is a legit and reputable on...
buy crystal methamphetamine
buy crystal methamphetamine Seller: 727267 Blacknetsales | buy crystal methamphetamine online blacknetsales.com is a legit and reputable on...
100mL Personal Lubricant Warming Based
100mL Personal Lubricant Warming Based Seller: Health & Beyond Inc. 100mL Personal Lubricant Warming Based Volume:100ml Our warming-based formula is enriched with ...
Synbio Technologies PCR cloning and Subcloning Technology
Synbio Technologies PCR cloning and Subcloning Technology Seller: Synbio Technologies PCR cloning andsubcloningtechnology, first developed in the 1970s, is now a staple in every mol...
Synthetic Biology Definition
Synthetic Biology Definition Seller: Synbio Technologies Synthetic biologydefinitionrefers to the design and construction of novel biological components, ...