TSEN2 Antibody
Product Name:TSEN2 AntibodyCode :CSB-PA850858DSR2HUSize :100μl:US$167 50μl:US$100Uniprot NO. :Q8NCE0Storage :Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.Image :Western blot All lanes:TSEN2 antibody at 5ug/ml+293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size :53kDa Observed band size :53kDa Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA850858DSR2HU at dilution 1:100Immunogen :Recombinant human tRNA-splicing endonuclease subunit Sen2 protein (1-220aa)Raised in :RabbitSpecies Reactivity :HumanTested Applications:ELISA,WB,IHC;Recommended dilution:WB:1:200-1000,IHC:1:50-1:200Relevance :Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.Form :LiquidConjugate :Non-conjugatedStorage Buffer :PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Purification method :Antigen Affinity PurifiedIsotype :IgGClonality :PolyclonalAlias :tRNA-intron endonuclease Sen2,HsSen2,TSEN2,SEN2Species :Human
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