Rat Anti-Smooth Muscle Antibody,ASMA ELISA Kit
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Product Name:
Rat Anti-Smooth Muscle Antibody,ASMA ELISA Kit
Product Type :
ELISA Kit
Code :
CSB-E08794r
Size :
96T,5×96T,10×96T
Abbreviation :
Anti-Smooth Muscle Antibody,ASMA
Species :
Rat
Detect Range :
0.16 ng/ml-10ng/ml
Sensitivity :
0.04 ng/ml
Assay Time :
1-5h
Sample Volume :
50-100ul
Detection Wavelengt :
450 nm
Availability :
3-5 working days
Research Area :
Signal Transduction
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of rat ASMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
93
Range %
88-99
1:2
Average %
96
Range %
90-103
1:4
Average %
99
Range %
96-104
1:8
Average %
102
Range %
96-106
Recovery :
The recovery of rat ASMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
92
89-98
EDTA plasma (n=4)
98
95-103
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
10
2.262
2.390
2.326
2.127
5
1.714
1.641
1.678
1.479
2.5
1.124
1.211
1.168
0.969
1.25
0.687
0.724
0.706
0.507
0.62
0.466
0.480
0.473
0.274
0.31
0.348
0.330
0.339
0.140
0.16
0.260
0.250
0.255
0.056
0
0.203
0.194
0.199
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