Pig Glial Cell Line-derived Neurotrophic Factor (GDNF) ELISA Kit
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Product Name:
Pig glial cell line-derived neurotrophic factor (GDNF) ELISA kit
Product Type :
ELISA Kit
Code :
CSB-EL009356PI
Size :
96T,5×96T,10×96T
Uniprot NO. :
D2KMN4
Abbreviation :
GDNF
Alias :
ATF1, ATF2, HFB1-GDNF, astrocyte-derived trophic factor|glial cell line derived neurotrophic factor
Species :
Pig
Sample Types :
serum, plasma, tissue homogenates
Sensitivity :
0.06 ng/ml
Assay Time :
1-5h
Sample Volume :
50-100ul
Detection Wavelengt :
450 nm
Availability :
3-5 working days
Research Area :
Neuroscience
Target details :
This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.
HGNC :
32789
RGD :
1565220
MGI :
3607786
BIOWWW :
GDNF
Precision :
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of pig GDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 105 Range % 103-109 1:2 Average % 97 Range % 91-102 1:4 Average % 91 Range % 87-96 1:8 Average % 94 Range % 89-98
Recovery :
The recovery of pig GDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 103 96-107 EDTA plasma (n=4) 92 87-95
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 16 2.033 2.111 2.072 1.955 8 1.781 1.912 1.847 1.730 4 1.464 1.511 1.488 1.371 2 1.155 1.211 1.183 1.066 1 0.829 0.801 0.815 0.698 0.5 0.475 0.474 0.475 0.358 0.25 0.253 0.266 0.260 0.143 0 0.121 0.112 0.117
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