Fmoc / t-Bu Solid Phase Synthesis
The fmoc ltdmethod is a new method for solid phase synthesis of peptides developed by Carpino and Hart based on the Boc method.
The fundamental difference between the Fmoc/t-Bu(9-fluorenylmethoxycarbonyl/tert-butoxy) strategy and the Boc/Bzl strategy is the use of alkali-removable Fmoc is a protecting group for α-amino group, the side chain is protected by t-Bu which can be removed by TFA, and the solid phase carrier Wang resin with acid-sensitive linking arm is used, and the final step of the synthesis is removed by TFA. Han Xiang et al. successfully synthesized the 32-peptide thymosin α 1 by Fm oc solid phase method, starting from Fm oc-Asn(Trt)-W angResin, Boc protecting the side chain amino group of Lys, and tert-butyl ester group (O-t-Bu Protecting the side chain carboxyl group of Asp and Glu, t -Bu protects the side chain hydroxyl group of Ser and Thr, and trityl group (Trt) protects the side chain amide group of Asn, and the total synthesis yield is 33.2%, and the purity is >98.8%.
The Fmoc protected amino acid is sensitive to bases, especially secondary amines, and can be removed with piperidine at room temperature. Under these conditions, Z-based, Boc-based or other amino protecting groups are unaffected.
The Fmoc protecting group is stable to acid and can be removed under mild alkaline conditions. It can be used orthogonally to acid-sensitive side chain protecting groups, and has the advantages of mild reaction conditions, less side reactions, and high yield.
Purification of the product is also facilitated when the polyaryl analog of the Fmoc protecting group is attached to the N-terminus of the polypeptide and protein. At the same time, the Fmoc group has characteristic ultraviolet absorption and is easy to monitor and control the reaction, so it is more and more popular.
Moreover, with the development of the peptide synthesis industry, the cost of Fmoc to protect amino acid raw materials is greatly reduced, and the Fmoc method has gradually replaced the Boc method and is widely used in solid phase synthesis.
Sunresin is an innovation oriented high-tech enterprise. It is specialized in R&D, manufacturing and sales of adsorption and separation polymer resins, equipments and customized solutions including support services. As the first and the only A-share listed company in chinese ion learning exchangeand Adsorption Resin Industry, Sunresin manufactures about 50,000 cubic meters of ion exchange and adsorption resins annually, and supplies customers with tens of adsorption and separation equipment.
Send product request
Other supplier products
 |
Bio-Pharmaceutical & Life Science | Antibiotice purification; Peptide antibiotic synthesis; Enzyme-catalyzed reaction; Chromatographic separation Modern biopharmaceutical technologie... |
 |
Mixed Bed Ion Exchange Resin | SEPLITE ® Mixed Bed Ion Exchange Resin Mixed bed is typically cation and anion ion exchange resin all in one mixture. Mixed bed ion exchange ... |
 |
Ion Exchange Resins | SEPLITE ® Ion Exchange Resins Want to know about ion exchange resin deutsch, don’t hesitate to contact us. Ion exchange is a powerful che... |
 |
Enzyme Carriers | SEPLITE ®Enzyme Carriers Enzyme carrier is a kind of Resin beads for Enzyme immobilization. The resin enzymecatalysis technology mainly relie... |
 |
PAM Resin | 4-Hydroxymethyl-phenylacetamidomethyl resin PAM resinhas become the most widely used resin in Boc chemistry peptide synthesis. It has greater acid... |
Same products
 |
Pleasure Lifting Liquid Sex Toy Room Products Fun Women Pleasure Lifting Liquid | Seller: AmorSerere | **Product Name:** Pleasure Lifting Liquid Sex Toy Room Products Fun Women Pleasure Lifting ... |
 |
Custom Eco-Friendly Personal Lubricant Cylinder Packaging Luxury Kraft Lube OEM | Seller: AmorSerere | **Product Name:** Custom Eco-Friendly Personal Lubricant Cylinder Packaging Luxury Kraft Lu... |
 |
Factory Organic Super Drawing Water-Based Lubricant Enhanced Pleasure Personal Lube | Seller: AmorSerere | **Product Name:** Factory Organic Super Drawing Water-Based Lubricant Enhanced Pleasure Per... |
 |
OEM 60ML Menstrual Cup and Menstrual Disc Lube Gel Personal Lubricant | Seller: AmorSerere | **Product Name:** OEM 60ML Menstrual Cup and Menstrual Disc Lube Gel Personal Lubricant**Mo... |
 |
Flavored Lube 200ml Personal Lubricants Sex Lubricant Flavour Gel | Seller: AmorSerere | **Product Name:** Flavored Lube 200ml Personal Lubricants Sex Lubricant Flavour Gel**Model ... |
