EPPS BUFFER CAS NO.16052-06-5
EPPS BUFFER CAS NO.16052 06 5
The biological EPPS buffer is a kind of 4 2 hydroxyethyl 1 piperazinepropanesulphonic acid that is one of the most widely used auxiliary reagents in biochemical experimental research. It mainly plays the role of adjusting the pH value of the reaction environment. Most biochemical reactions are originally carried out in vivo, and usually require The catalysis of the enzyme is regulated, and the enzyme activity needs to be expressed at a suitable pH. Organisms have their own systems to adjust the pH, but in vitro experiments need to use a buffer such as EPPS buffer to maintain a proper pH.
EPPS Buffer Details
EPPS is a commonly used Good's buffer with the same properties as other Good's buffers and is widely used in biological research. It is often used as a separator in ultrathin isoelectric focusing gels and improves the resolution of phosphoglucomutase. Rate. In addition, it is also commonly used as a buffer to adjust the pH value in many experiments.
In biological experiments, it is important to maintain the pH of the solutions used. Usually buffers, mixtures of appropriate weak acids and their conjugate bases are used. Most biological reactions take place at a neutral pH of 6 to 8. The buffer needs to be valid within this range. In addition, the acids and bases used in buffers should not form chelates with metal ions, which are essential in biological systems. EPPS is a zwitterionic buffer specially developed for biological research and analysis.
Desheng's Advantages in EPPS Buffer
The product has stable performance and is jointly produced by the company's internal professional R&D team;
Pure white appearance, purity up to 99%;
The price of the source manufacturer is more advantageous, and the larger quantity is more discounted;
Possess a wealth of technical knowledge reserves and can provide efficient technical support;
As a production-type manufacturer, Desheng Company has favorable product prices compared with those in the same industry;
Professional after-sales service team, each customer has personnel tracking and responsible to ensure that customer problems can be solved in time;
The company has cooperated with major express logistics companies, which can quickly, conveniently and safely deliver goods to customers in a timely manner.
Product attribute advantages: high water solubility; low cell membrane permeability; consistent acid-base dissociation constant; low metal chelation ability; high chemical stability; low absorption spectrum in the ultraviolet and visible light regions.
Application of EPPS Buffer
As a buffer system commonly used in DNA screening systems;
It can also be used as a buffer component of RNA samples;
It is suitable for electron transfer and chemical research of chloroplast thin layer preparation, and protects oxyhemoglobin from oxidation to methemoglobin during freeze-drying;
It can also be used as a background electrolyte for micro-analysis of proteins by capillary zone electrophoresis;
Determination of protein content by Folin phenol method.
Instructions for use of EPPS Buffer
The preparation method of EPPS buffer is similar to that of HEPES and BICINE. The pH value is adjusted with sodium hydroxide lye, and the buffer solution with specific pH value can be obtained by mixing in proportion. If a more accurate pH value is required, it is necessary to Measure the pH and adjust the mixing ratio as needed.
It should be noted that the preparation temperature is controlled at about 20 degrees; if the detection environment needs to exclude sodium ions, it is also possible to use potassium hydroxide instead of sodium hydroxide, and the weight can be changed according to the corresponding molecular weight.
The preparation method of EPPS buffer:
(1) with 0.1M solution (A): EPPS25.233g/deionized water 1,000ml;
(2) with 0.1M NaOH solution (B): NaOH 4g/deionized water 1,000ml;
(3) Mix the two solutions A and B in proportion and adjust according to the pH value.
EPPS Buffer Precautions
Since the appearance of EPPS buffer is powder, it is easy to absorb moisture when stored, and the remaining powder should be strictly sealed and stored away from light after use;
Due to the different production process, it is not advisable to mix it with other companies' EPPS products to avoid losses;
When configuring, clean the container more than three times to avoid material exchange with other products;
Wear gloves and other protective equipment when using to avoid damage to the skin.
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