L-Cystine dihydrochloride Price
L-Cystine dihydrochloride is the diprotonated dihydrochloride salt of L-cystine, formed by the reaction of L-cystine with 2 equivalents of HCl. Its chemistry is dominated by:
the disulfide bond (–S–S–), protonated amino groups, carboxyl groups, and salt structure.
1. Acidic Property
- Contains two protonated –NH₃⁺ groups and two carboxyl groups (–COOH).
- Aqueous solution is strongly acidic:
- 1% solution/water suspension: pH ≈ 1.0–2.0
- Reacts with strong bases (NaOH, KOH, ammonia water) to form salts and gradually release free L-cystine, which precipitates as pH rises.
2. Stability & Reactivity of the Disulfide Bond
The –S–S– bond is the most chemically active site:
(1) Reduction reaction
Disulfide bond can be cleaved by reducing agents to form two molecules of L-cysteine:
- Reducing agents: NaBH₄, dithiothreitol (DTT), β-mercaptoethanol, Zn/H⁺, H₂/Pd
- Reaction:
(2) Oxidation reaction
Under strong oxidizing conditions (H₂O₂, concentrated HNO₃, KMnO₄),
the disulfide bond is oxidized to sulfinic acid or sulfonic acid derivatives, and the carbon skeleton may be broken.
(3) Disulfide exchange
Reacts with other thiol compounds (cysteine, glutathione) to form mixed disulfides.
3. Salt-forming and Neutralization Reactions
- Reacts with strong bases to neutralize HCl and deprotonate the ammonium groups:
- At low pH: forms mono-salt
- At neutral/weak alkaline pH: precipitates free L-cystine (very low solubility)
- Reacts with silver, lead, mercury salts to form sulfur-containing metal precipitates.
4. Hydrolytic Stability
- Stable to dilute acid at room temperature.
- Under strong acid hydrolysis (6 M HCl, 110 °C), the skeleton remains stable, but partial oxidation may occur under aerobic conditions.
- In strong alkaline hydrolysis, disulfide bonds are easily broken and sulfur may be lost as H₂S.
5. Optical Activity
- Has chiral centers; exhibits stable optical rotation.
- Specific rotation [α]D20 ≈ +225° ~ +240° (in 1 M HCl)
- Racemization occurs only under strong alkali, high temperature, or strong catalytic conditions.
6.Decarboxylation & Thermal Decomposition
- When heated strongly (above ~220 °C), it decomposes rather than melts cleanly.
- Decomposition products may include: CO₂, H₂S, NH₃, thiols, and charred residues.
7. Compatibility
- Incompatible with strong oxidizing agents (causes decomposition, gas release).
- Incompatible with strong bases (causes precipitation of free cystine).
- Slightly hygroscopic but stable under dry, airtight conditions.
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