Human Interferon β(IFN-β/IFNB)ELISA Kit
ThiskitisusedtoassaytheInterferonβ(IFN-β/IFNB)inthesampleofHuman’sserum,bloodplasmaandotherrelatedbiologicalliquid.
Thiskitusesenzyme-linkedimmunesorbentassay(ELISA)basedontheBiotindoubleantibodysandwichtechnologytoassaytheHumanInterferonβ(IFN-β/IFNB).AddInterferonβ(IFN-β/IFNB)tothewells,whicharepre-coatedwithInterferonβ(IFN-β/IFNB)monoclonalantibodyandthenincubate.Afterthat,addantiIFN-β/IFNBantibodieslabeledwithbiotintounitewithstreptavidin-HRP,whichformsimmunecomplex.Removeunboundenzymesafterincubationandwashing.AddsubstrateAandB.Thenthesolutionwillturnblueandchangeintoyellowwiththeeffectofacid.TheshadesofsolutionandtheconcentrationofHumanInterferonβ(IFN-β/IFNB)arepositivelycorrelated.
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