CytoLinX® WB Single-Use Rocking Bioreactor
CytoLinX® WB Single Use Rocking Bioreactoris available in a variety of models, including 10 L, 20 L, and 50 L for 0.3–25 L culture volumes, and 100 L and 200 L for 10–100 L culture volumes. During the culture process, introduce air/oxygen (filtered by a ster-ile filter) into the culture bag to obtain a certain space. Place the culture bag on the rocking platform to rock, and the medium will form a rocking movement in the culture bag. Such design enables good oxygen supply and cell mixing, as well as a mild cell culture microenvironment with low shear force and high dissolved oxygen. This improves the cell status by protecting them from damage due to the shear force and bubbling at the impeller ends in traditional mixing vessels. As a result, the undulating fluid level of the medium is constantly in contact with the oxygen introduced into the culture bag, providing sufficient dissolved oxygen for cell growth. Cell density and product yield can be increased by adjusting the shaking speed and angle, and by in-troducing a certain ratio of air/oxygen mixture into the wave bioreactor bags. Featured by flexible culture volume range, easy operation, precise and reliable control, and easy process scale-up, the rocking bioreactor is suitable for various cell culture conditions, including scientific research, research and development, in-process seed culture, and new therapies, such as cell therapy.
The rocking bioreactor works with pre-irradiated sterile cell culture bags, eliminating the risk of contamination and avoiding bioreactor cleaning and validation, thus shortening process development and production cycles. The use of a sterile cell cul-ture bag increases the success rate of cell culture, and the closed culture system avoids direct contact between the feed liquid and the operator so as to ensure biological safety.
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