Nisin in Beverages

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Application in Beverages: Controlling Heat-Resistant Spores and Microbial Growth
Beverages (e.g., fruit juices, beer, functional drinks) face risks of spoilage by heat-resistant spores (e.g., Alicyclobacillus in acidic juices) and bacteria (e.g., Lactobacillus in beer). Nisin’s stability in acidic environments (pH 2.0–6.0) makes it suitable for beverage preservation:
Key Applications & Mechanisms
Fruit juices (orange juice, apple juice):
Method: Add 0.05–0.1 g/kg Nisin to juice after pasteurization, or combine with high-pressure processing (HPP, 600 MPa for 5 minutes).
Effect: Inhibits Alicyclobacillus spores (which cause off-flavors like "medicinal taste" in acidic juices). Pasteurized orange juice with Nisin has a shelf life extended from 30 days to 60–90 days at room temperature, with no loss of vitamin C.
Beer and wine:
Method: Add 0.01–0.03 g/kg Nisin to beer before bottling, targeting lactic acid bacteria (which cause "souring" of beer).
Effect: Prevents bacterial spoilage without affecting beer’s foam stability or flavor. Craft beer with Nisin maintains its original taste for 6–9 months, compared to 3–4 months without Nisin.
Advantage
Avoids the need for ultra-high-temperature (UHT) sterilization, which destroys beverage aromas (e.g., fresh fruit notes in juices).

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