SPE CARTRIDGES

RayCure SPE series provides high-quality SPE cartridgesfor various types of solid phase extraction, including manifold, vacuum as well as automated solid phase extraction equipment, well compatible with RayKol automated solid-phase extraction systems. RayCure SPE cartridges utilize high-quality sorbents to ensure the efficiency of SPE clean up, offers full range of solid phase extraction cartridgefor various sample matices. Strict quality managment and control ensures high recovery rate of analytes and experiment reproducibility. RayKol provides effective, efficient and economial SPE cartridges with high performance.

SPE CARTRIDGE TYPES

RayCure SPE Column

RayCure SPE series provide pre-packed SPE columns with a variety of sorbents, resins and cartridge size for various methods and target analytes.

RULES TO SELECT RAYCURE SPE CARTRIDGE TYPES

Rule I:

The properties of solid phase packing in SPE cartridges should be match to target compounds.

Rule II:

Take the advantage of differences between analytes of interest and interferences

Rule III:

Consider nature of SPE sample matrices, characteristics of analytes and solubility of used solvents

Users can select RayCure SPE columns depending on properties of desired analytes and sample matrices as following selection guides.

Target compounds	Properties of analytes		Corresponding SPE
	Solubility	Polarity/Ionicity	SPE mechanism	Sorbent phase
	Water soluble	Compounds with high/low polarity	Non-polar	C18
			Polymer	HLB
		Ionic compounds	Anion exchange	MCX, WCX
			Cation exchange	MAX, WAX
	Soluble in organic solvents	Polar compounds	Normal phase bonding	PSA, NH2
		Compounds with neutral polarity	Normal phase adsorption	Silica, Florisil
		Non-polar compounds	Reversed phase	C18

Based on nature of SPE sample matrices

SPE sample matrices

SPE sample matrices	Target compounds	Impurities	RayCure SPE column
Fruits, vegetables, fruit juice, vegetable juice, fruit wine, herbs	Multiple pesticide residues	Carbohydrates, pigments, organic acid, phenol	RayCure QuEChERS d-SPE kits
			RayCure NH2
			RayCure PSA
			RayCure GCB/NH2
			RayCure GCB/PSA
			RayCure Florisil
	Weak polar pesticides	Carbohydrates, pigments, organic acid, phenol	RayCure HLB
			RayCure C18
	Alkali pesticides	Carbohydrates, pigments, organic acid, phenol	RayCure MCX
			RayCure WCX
	Acidic pesticides	Carbohydrates, pigments, organic acid, phenol	RayCure MAX
			RayCure WAX
Dairy products, blood, urine, animal tissues	Neutral, weak alkali, weak acidic drugs	Proteins, fats	RayCure HLB
			RayCure C18
	Alkali drugs	Proteins, fats	RayCure MCX
			RayCure WCX
	Acidic drugs	Proteins, fats	RayCure MAX
			RayCure WAX
Soil, wastewater	hydrophobic organic pollutants	Humic substances	RayCure HLB
			RayCure C18
	Pesticide residues	Humic substances	RayCure Florisil
Oil and grease	Fat-soluble vitamins, phospholipids, aflatoxins	Fats	RayCure Silica
			RayCure NH2
			RayCure PSA

HOW DOES SPE COLUMN WORK?

SPE is a conventional and efficient extraction technique, it uses the affinity of solutes dissolved or suspended in mobile phase for stationary phase to separate desired compounds from a mixture. And there are two solid phase extraction mechanisms to obtain target analytes from complex samples, to retain desired compounds in stationary phase or retain impurities in solid phase. Depending on the difference of two mechanisms, SPE procedures could be a bit various.

When sample portion passes through SPE column, undesired impurities are discarded with mobile phase, target compounds are retained in stationary phase, then rinse stationary phase with eluents to collect target compounds. It is the most commonly used purification mechanism of solid phase extraction.

In the opposite, to retain impurities in stationary phase, target compounds and few impurities are collected in mobile phase when sample portion passes through SPE column. Rinse the stationary phase which impurities is retained with solvents to obtain rest of retained target compounds. This mechanism is mostly used to eliminate fat-soluble impurities of ion analysis and analysis of multiple pesticides in fruits and vegetables.

For more information about total laboratory automation systemand automated liquid handler, please feel free to contact us!

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