Gentamicin ELISA Test Kit
Gentamicin ELISA Test Kit
Catalog No. LSY-10023
This test kit is based on the competitive enzyme immunoassay for the detection of Gentamicin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Gentamicin in the sample and pre-coated coupling antigen on the micro-well stripes compete for the anti-Gentamicin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Gentamicin in it. The value is compared to the standard curve and the Gentamicin concentration is subsequently obtained.
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 60min～15min
Detection limit
Chicken, Liver................................................................................... 4 ppb
Cross-reaction rate
Gentamicin........................................................................................ 100%
Streptomycin...................................................................................... <1%
Dihydrostreptomycin........................................................................... <1%
Neomycin........................................................................................... <1%
Recovery rate
Chicken, Liver................................................................................ 70±18%
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb
3） Enzyme conjugate (7 mL)............................................................... red cap
4） Antibody working solution (7 mL).................................................. blue cap
5） Substrate A solution (7 mL).......................................................... white cap
6） Substrate B solution (7 mL)......................................................... black cap
7） Stop solution (7 mL).................................................................. yellow cap
8） 20× concentrated washing buffer (40 mL)..................................... white cap
9） 2× concentrated redissolving solution (50 mL)..................... transparent cap
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