Benzyl penicillin ELISA Test Kit

Benzyl penicillin ELISA Test Kit

Catalog No. LSY-10019

This test kit is based on the competitive enzyme immunoassay for the detection of Benzyl penicillin in samples. The antigens conjugated Benzyl penicillin is pre-coated on the micro-well stripes, Benzyl penicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Benzyl penicillin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Benzyl penicillin concentration in the sample. This value is compared to the standard curve and concentration of Benzyl penicillin in the sample is subsequently obtained.

 

Sensitivity: 0.1 ppb

Incubator temperature:  25℃

Incubator time:  30min~30min~15min

Detection limit:

Tissue, honey, milk ........................................................................... 2 ppb

Because of some interference, quantification limit of honey, milk sample is 4 ppb

Recovery rate:

Tissue............................................................................................ 75±19%

Honey, milk.................................................................................... 70±17%

Cross-reaction rate:

Benzyl penicillin................................................................................. 100%

Ampicillin........................................................................................... 0.8%

Cloxacillin........................................................................................... 0.2%

Dicloxacillin........................................................................................ 0.1%

Amoxicillin.......................................................................................... 0.1%

Ceftiofu.............................................................................................. 0.1%

 

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb

3)   Enzyme conjugate (12 mL)............................................................. red cap

4)   Antibody working solution (7 mL).................................................. blue cap

5)   Substrate A solution (7 mL).......................................................... white cap

6)   Substrate B solution (7 mL)......................................................... black cap

7)   Stop solution (7 mL).................................................................. yellow cap

8)   20× concentrated washing buffer (40 mL)..................................... white cap

9)   5× concentrated redissolving solution (50 mL)..................... transparent cap




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