Safety Considerations in Practical Applications of Nisin

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Dosage Control: Excessive addition (>0.5 g/kg) not only increases production costs but may also cause slight bitterness in foods (e.g., dairy products, beverages) due to high peptide concentration, though it does not pose health risks. Strict adherence to national dosage limits is required to balance efficacy and sensory quality.
Special Populations: While Nisin is generally safe for infants and pregnant women, its use in infant foods (<36 months) requires compliance with specific standards (e.g., China’s GB 2760 stipulates a maximum of 0.1 g/kg in infant formula accessories).
Cross-Contamination: Nisin production facilities should be separated from those producing allergens (e.g., milk, soy) to avoid cross-contamination, though the risk is inherently low due to its non-allergenic nature.

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