Sulfonamides residues (SAs) ELISA Test Kit
Sulfonamides (SAs) ELISA Kit Catalog No. LSY-10009 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Sulfonamides in the chicken, pork, milk, honey, egg, etc. The coupling antigens are pre-coated on the micro-well stripes. The Sulfonamides in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfonamides antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfonamides in the sample. This value is compared to the standard curve and the Sulfonamides concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.4 ppb Detection limit Tissue (high-detection-limit method) 0.4 ppb Tissue (lower-detection-limit method)……………………………………...2 ppb Honey, egg …………………………………………………………………0.4 ppb Serum, urine 1.6 ppb Milk 8 ppb Recovery rate Tissue, honey 90 ±10% Egg 65 ±10% Urine, milk, serum 70 ±10% Cross-reaction rate Sulfadiazine (SD or SDZ) 100% Sulfamonomethoxine (SMM) 97.6% Sulfamethoxazole (SMZ) 100% Sulfathiazole (ST) 195.0% Sulfamerazine (SM1) 92% Phthalylsulfathiazole (PST) 73% Sulfamethyltiadiazolum 110% Sulfapyridine 48% Sulfamethoxydiazine(SMD) 26.4% Sulfafurazole(SIZ) 95.0% Sulfanethazine(SM2)………………………………………………………. 140% Sulfadimethoxine(SDM)…………………………………………………… 110% Sulfadoxine (SDM2)…………………………………………………………..80% Sulfaquinoxaline(SQX)………………………………………………………160% Sulfamethoxypyridazine (SMP)………………………………………………80% Sulfachlorinepyridazine (SPDZ)……………………………………………...75% Sulfabenzoyl (SML)…………………………………………………………..104% Conclusion drawn from the cross-reaction rate: This kit can be used for the test of Sulfonamides, totally meet the national testing demand of Sulfonamides. 3. Components 1） Micro-well strips: 12 strips with 8 removable wells each 2） 6× standard solution (1 mL each): 0 ppb, 0.4 ppb, 1.2 ppb, 3.6 ppb, 10.8 ppb and 32.4 ppb 3） Enzyme conjugate (7 mL) red cap 4） Antibody working solution (10 mL) blue cap 5） Substrate A solution (7 mL) white cap 6） Substrate B solution (7 mL) black cap 7） Stop solution (7 mL) yellow cap 8） 20× concentrated washing buffer (40 mL) white cap 9） 2× concentrated redissolving solution (50 mL) transparent cap
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