Nitrofuran (SEM) ELISA Test Kit

Nitrofuran (SEM) ELISA Test Kit

Catalog No. LSY-10004

This test kit is based on the competitive enzyme immunoassay for the detection of Nitrofuran (SEM) in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Nitrofuran (SEM) in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SEM in it. This value is compared to the standard curve and the SEM concentration is subsequently obtained.

Sensitivity:  0.02 ppb

Incubation Temperature:  25

Incubation Time:  30min15min

Cross-reaction rate

SEM································································································ 100%

AMOZ··························································································· < 0.1%

AOZ······························································································ < 0.1%

AHD······························································································ < 0.1%

Recovery rate

Tissue·························································································· 75±25%

Honey·························································································· 70±20%

1)    Micro-well strips: 12 strips with 8 removable wells each

2)    6× standard solution (1 mL each): 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb and 1.62ppb

3)    Enzyme conjugate (7 mL)······························································ red cap

4)    Antibody working solution (7 mL)·················································· blue cap

5)    Substrate A solution (7 mL)························································· white cap

6)    Substrate B solution (7 mL)························································ black cap

7)    Stop solution (7 mL)································································· yellow cap

8)    20× concentrated washing buffer (40 mL)···································· white cap

9)    2× concentrated redissolving solution (50 mL)···················· transparent cap

10)   2-Nitrobenzaldehyde (10 mL)······················································· white cap




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