Nitrofuran (SEM) ELISA Test Kit
Nitrofuran (SEM) ELISA Test Kit
Catalog No. LSY-10004
This test kit is based on the competitive enzyme immunoassay for the detection of Nitrofuran (SEM) in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Nitrofuran (SEM) in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SEM in it. This value is compared to the standard curve and the SEM concentration is subsequently obtained.
Sensitivity: 0.02 ppb
Incubation Temperature: 25℃
Incubation Time: 30min～15min
Cross-reaction rate
SEM································································································ 100%
AMOZ··························································································· < 0.1%
AOZ······························································································ < 0.1%
AHD······························································································ < 0.1%
Recovery rate
Tissue·························································································· 75±25%
Honey·························································································· 70±20%
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb and 1.62ppb
3) Enzyme conjugate (7 mL)······························································ red cap
4) Antibody working solution (7 mL)·················································· blue cap
5) Substrate A solution (7 mL)························································· white cap
6) Substrate B solution (7 mL)························································ black cap
7) Stop solution (7 mL)································································· yellow cap
8) 20× concentrated washing buffer (40 mL)···································· white cap
9) 2× concentrated redissolving solution (50 mL)···················· transparent cap
10) 2-Nitrobenzaldehyde (10 mL)······················································· white cap
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