Oxytetracyline ELISA Test Kit

Oxytetracyline ELISA Test Kit

Catalog No. LSY-10044

This test kit is based on the competitive enzyme immunoassay for the detection of Oxytetracyline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Oxytetracylinein the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Oxytetracyline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Oxytetracyline in it. This value is compared to the standard curve and the Oxytetracyline concentration is subsequently obtained.

 

Sensitivity: 0.4 ppb

Incubation Temperature: 25

Incubation Time:30min—15min

Detection limit:

Tissue..................................................................................... about 6ppb

Cross-reaction rate:

Oxytetracyline.................................................................................. 100%

Tetracyclines.................................................................................... 200%

Chlortetracycline............................................................................... 200%

Doxycycline...................................................................................... 20%

Recovery rate:

Tissue ......................................................................................... 90±15%

 

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   10× concentrated standard solution: 0 ppb, 4ppb, 12ppb, 36ppb, 108ppb

3)   Enzyme conjugate (7 mL)

4)   Antibody working solution (7 mL)

5)   Substrate A (7 mL)

6)   Substrate B (7 mL)

7)   Stop solution (7 mL)

8)   20× concentrated washing buffer (30 mL)

9)   20× concentrated redissolving solution (10 mL) 




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