Dexamethasone ELISA test Kit
Dexamethasone ELISA test Kit Catalog No. LSY-10042 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Dexamethasone in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Dexamethasone in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Dexamethasone antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Dexamethasone in it. This value is compared to the standard curve and the Dexamethasone concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.1ppb Incubation Temperature: 25℃ Incubation Time: 30min ～ 15min Detection limit: Tissue 0.1 ppb Feed 0.2 ppb Milk powder 0.3 ppb Milk 0.2 ppb Honey 0.2 ppb Urine 0.2 ppb Cross-reaction rate: Dexamethasone 100% Recovery rate Tissue, feed 90±25% Milk powder 85±25% Urine, milk, honey…………………………………………………………. 95±25% 3. Components 1） Micro-well strips: 12 strips with 8 removable wells each 2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb 3） Enzyme conjugate (7 mL) red cap 4） Antibody working solution (7 mL) blue cap 5） Substrate A solution (7 mL) white cap 6） Substrate B solution (7 mL) black cap 7） Stop solution (7 mL) yellow cap 8） 20× concentrated washing buffer (15 mL) white cap 9） 5× concentrated redissolving solution (110 mL) transparent cap
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