Human Amyloid β Protein 40 ELISA kit
E01A0049 Human Amyloid β Protein 40 ELISA kit
Human Adenosine diphosphate ELISA kit is suitable for the detection of samples from human species. Amyloid Beta ELISAProtein 40 can also be called Aβ40, beta-amyloid 40.
Principle of the Assay
AΒ40 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AΒ40. Standards or samples are then added to the microtiter plate wells and AΒ40 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AΒ40 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AΒ40 are added to each well to “sandwich” the AΒ40 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AΒ40 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AΒ40 concentration in each sample is interpolated from this standard curve.
Quality Control on Human Amyloid β Protein 40 ELISA kit
|
Coefficient of Variance |
Intra Variation% ＜10% |
|
|
Inter Variation% ＜12% |
||
|
Recovery |
92-105% |
|
|
Linearity |
Diluent Ratio |
Range % |
|
1:02 |
96-105 |
|
|
1:04 |
903-103 |
|
|
1:08 |
89-107 |
|
|
Specificity/Cross-reactivity |
No significant cross-reactivity or interference between AΒ40 and analogues was observed. |
|
Citations of Human Amyloid β Protein 40 ELISA kit
E01A0049 has been referenced in the below publications:
Therapeutic effect of probucol on mild cognitive impairment in type 2 diabetic patients.
Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer's Disease-Like Amyloid-β Pathology and Neuroinflammation in the Rat Hippocampus.
Elisa kit supplier Shanghai BlueGeneBiotech is a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio®" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit.
If you want to know more kinds of bovine igg elisa kits, please visit our website.
Send product request
Other supplier products
 |
Human/Canine/Porcine Insulin ELISA kit | NE01I0004 Human/Canine/Porcine elisa insulin kit Human/Canine/Porcine elisa for insulinkit is suitable for the detection of samples from human s... |
 |
Mouse ELISA Kits | Laboratory mouse makes an ideal model for human disease because the organization of their DNA and their gene expression is similar to humans. They ... |
 |
BlueGene Biotech Porcine Total Adiponectin ELISA kit | BlueGene Biotech Porcine Total Adiponectin ELISAkit Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte ... |
 |
BlueGene Biotech Bovine Catalase ELISA kit | E11C0086 Bovine Catalase ELISA kit Bovine Catalase ELISA kitis suitable for the detection of samples from Bovine species. Catalase can also be c... |
 |
Human Adenosine Triphosphate ELISA kit | E01A0038 Human Adenosine Triphosphate ELISA kit Human Adenosine Triphosphate ATP ELISAkit is suitable for the detection of samples from human sp... |
Same products
 |
HPMC powder | Seller: YIDA HPMC | Hydroxypropyl methylcellulose (HPMC) is made of refined cotton after being pulverized, then react... |
 |
UHMWPE Plant | Seller: Hubei Sanli Fengxiang Technology Co., Ltd | Ultra high molecular weight polyethylene (hereinafter called as UHMWPE) is a linear structural po... |
 |
Formic acid production technology | Seller: Hubei Sanli Fengxiang Technology Co., Ltd | There are totally four production processes forformic acid production, incl. Sodium Formate Proce... |
 |
PPC Plant | Seller: Hubei Sanli Fengxiang Technology Co., Ltd | PPC (polypropylene carbonate)is acompletely biodegradable environment-friendly plastic synthesize... |
 |
Buy Caluanie Muelear Ship in Europe | Seller: CALUANIE MUELEAR OXIDIZE | Caluanie (Oxidative Partition Thermostat, Heavy Water) Appearance: Transparent liquid. Smell: Ord... |
