Human Amyloid β Protein 40 ELISA kit
E01A0049 Human Amyloid β Protein 40 ELISA kit
Human Adenosine diphosphate ELISA kit is suitable for the detection of samples from human species. Amyloid Beta ELISAProtein 40 can also be called Aβ40, beta-amyloid 40.
Principle of the Assay
AΒ40 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AΒ40. Standards or samples are then added to the microtiter plate wells and AΒ40 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AΒ40 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AΒ40 are added to each well to “sandwich” the AΒ40 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AΒ40 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AΒ40 concentration in each sample is interpolated from this standard curve.
Quality Control on Human Amyloid β Protein 40 ELISA kit
Coefficient of Variance |
Intra Variation% <10% |
|
Inter Variation% <12% |
||
Recovery |
92-105% |
|
Linearity |
Diluent Ratio |
Range % |
1:02 |
96-105 |
|
1:04 |
903-103 |
|
1:08 |
89-107 |
|
Specificity/Cross-reactivity |
No significant cross-reactivity or interference between AΒ40 and analogues was observed. |
Citations of Human Amyloid β Protein 40 ELISA kit
E01A0049 has been referenced in the below publications:
Therapeutic effect of probucol on mild cognitive impairment in type 2 diabetic patients.
Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer's Disease-Like Amyloid-β Pathology and Neuroinflammation in the Rat Hippocampus.
Elisa kit supplier Shanghai BlueGeneBiotech is a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio®" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit.
If you want to know more kinds of bovine igg elisa kits, please visit our website.
Send product request
Other supplier products
BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit | E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit Canine Brain Derived Neurotrophic Factor BDNFELISA kitis suitable for the detection ... | |
BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit | E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detecti... | |
Human/Canine/Porcine Insulin ELISA kit | NE01I0004 Human/Canine/Porcine elisa insulin kit Human/Canine/Porcine elisa for insulinkit is suitable for the detection of samples from human s... | |
Human ELISA Kits | Our more than 8000 human visfatin elisa kitare intended to advance your research to study human disease covering several research areas (like cance... | |
BlueGene Biotech Guinea Pig Eosinophil Cationic Protein ELISA kit | E05E0125 Guinea Pig Eosinophil Cationic Protein ELISAkit Guinea Pig Eosinophil cationic protein ELISA kit is suitable for the detection of sampl... |