.

BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit

E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit

Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detection of samples from Bovine species. Cluster of Differentiation 8 can also be called P32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2,CD8.

Specifications of Bovine Cluster Of Differentiation 8 ELISA kit

Product Information

E11C0007

Product Name

Bovine Cluster of Differentiation 8 ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

Sensitivity

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C

Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial

Principle of the Assay

CD8 ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-CD8 antibody and an CD8-HRP conjugate. The assay sample and buffer are incubated together with CD8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition between CD8 from samples and CD8-HRP conjugate for the anti-CD8 antibody binding site, the intensity of the color is inversely correlated with the concentration of CD8. As more sites are taken up by CD8 from the sample, fewer sites are left that can bind CD8-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration.

Quality Control on Bovine Cluster Of Differentiation 8 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% 12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CD8 and analogues was observed.



Send product request

To: Bluegene Biotech
Your E-mail:
Message text:


Send to other suppliers

Other supplier products

BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E14A0081 Product Name Sheep Anti Inter...
BlueGene Biotech Canine Ferritin ELISA kit
BlueGene Biotech Canine Ferritin ELISA kit E08F0010 Canine Ferritin ELISA kit Canine Ferritin ELISAkit is suitable for the detection of samples from Canine species. Ferritin can also be c...
BlueGene Biotech Bovine Catalase ELISA kit
BlueGene Biotech Bovine Catalase ELISA kit E11C0086 Bovine Catalase ELISA kit Bovine Catalase ELISA kitis suitable for the detection of samples from Bovine species. Catalase can also be c...
Human Total β Amyloid Protein ELISA kit
Human Total β Amyloid Protein ELISA kit E01A0039 Human Total β Amyloid Protein ELISA kit Human Total β Amyloid Beta ELISA kitis suitable for the detection of samples from hum...
BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit
BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit Canine Brain Derived Neurotrophic Factor BDNFELISA kitis suitable for the detection ...
All supplier products

Same products

BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit Seller: Bluegene Biotech E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA...
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit Seller: Bluegene Biotech Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E1...
BlueGene Biotech Chicken Total Adiponectin ELISA kit
BlueGene Biotech Chicken Total Adiponectin ELISA kit Seller: Bluegene Biotech E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of ch...
BlueGene Biotech Bovine Decorin ELISA kit
BlueGene Biotech Bovine Decorin ELISA kit Seller: Bluegene Biotech E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of sam...
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit Seller: Bluegene Biotech E11C0339 Bovine C Type Natriuretic PeptideELISA kit Bovine C Type Natriuretic Peptide ELISAkit...