BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit
E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit
Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detection of samples from Bovine species. Cluster of Differentiation 8 can also be called P32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2,CD8.
Specifications of Bovine Cluster Of Differentiation 8 ELISA kit
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Product Information |
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E11C0007 |
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Product Name |
Bovine Cluster of Differentiation 8 ELISA kit |
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Species |
Bovine |
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Product Size |
48 Tests / 96 Tests |
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Concentration |
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Sensitivity |
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Principal |
Sandwich ELISA |
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Sample Volume |
50 ul |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Assay Time |
90 minutes |
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Platform |
Microplate Reader |
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Conjugate |
HRP |
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Detection Method |
Colorimetric |
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Storage |
2-8°C |
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Kit Components |
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MATERIALS |
SPECIFICATION |
QUANTITY |
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MICROTITER PLATE |
96 wells |
stripwell |
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ENZYME CONJUGATE |
10 mL |
1 vial |
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STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
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STANDARD B (0.5mL) |
1 vial |
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STANDARD C (0.5mL) |
5 ng/mL |
1 vial |
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STANDARD D (0.5mL) |
10 ng/mL |
1 vial |
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STANDARD E (0.5mL) |
25 ng/mL |
1 vial |
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STANDARD F (0.5mL) |
50 ng/mL |
1 vial |
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SUBSTRATE A |
6 mL |
1 vial |
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SUBSTRATE B |
6 mL |
1 vial |
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STOP SOLUTION |
6 mL |
1 vial |
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WASH SOLUTION (100 x) |
10 mL |
1 vial |
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BALANCE SOLUTION |
3 mL |
1 vial |
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Principle of the Assay |
CD8 ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-CD8 antibody and an CD8-HRP conjugate. The assay sample and buffer are incubated together with CD8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition between CD8 from samples and CD8-HRP conjugate for the anti-CD8 antibody binding site, the intensity of the color is inversely correlated with the concentration of CD8. As more sites are taken up by CD8 from the sample, fewer sites are left that can bind CD8-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration.
Quality Control on Bovine Cluster Of Differentiation 8 ELISA kit
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Coefficient of Variance |
Intra Variation% ＜10% |
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Inter Variation% ＜12% |
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Recovery |
91-105% |
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Linearity |
Diluent Ratio |
Range % |
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1:2 |
88-106 |
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1:4 |
87-108 |
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1:8 |
85-110 |
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Specificity/Cross-reactivity |
No significant cross-reactivity or interference between CD8 and analogues was observed. |
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