BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit
E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit
Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detection of samples from Bovine species. Cluster of Differentiation 8 can also be called P32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2,CD8.
Specifications of Bovine Cluster Of Differentiation 8 ELISA kit
|
Product Information |
|
|
E11C0007 |
|
|
Product Name |
Bovine Cluster of Differentiation 8 ELISA kit |
|
Species |
Bovine |
|
Product Size |
48 Tests / 96 Tests |
|
Concentration |
|
|
Sensitivity |
|
|
Principal |
Sandwich ELISA |
|
Sample Volume |
50 ul |
|
Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
|
Assay Time |
90 minutes |
|
Platform |
Microplate Reader |
|
Conjugate |
HRP |
|
Detection Method |
Colorimetric |
|
Storage |
2-8°C |
|
Kit Components |
||
|
MATERIALS |
SPECIFICATION |
QUANTITY |
|
MICROTITER PLATE |
96 wells |
stripwell |
|
ENZYME CONJUGATE |
10 mL |
1 vial |
|
STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
|
STANDARD B (0.5mL) |
1 vial |
|
|
STANDARD C (0.5mL) |
5 ng/mL |
1 vial |
|
STANDARD D (0.5mL) |
10 ng/mL |
1 vial |
|
STANDARD E (0.5mL) |
25 ng/mL |
1 vial |
|
STANDARD F (0.5mL) |
50 ng/mL |
1 vial |
|
SUBSTRATE A |
6 mL |
1 vial |
|
SUBSTRATE B |
6 mL |
1 vial |
|
STOP SOLUTION |
6 mL |
1 vial |
|
WASH SOLUTION (100 x) |
10 mL |
1 vial |
|
BALANCE SOLUTION |
3 mL |
1 vial |
|
Principle of the Assay |
CD8 ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-CD8 antibody and an CD8-HRP conjugate. The assay sample and buffer are incubated together with CD8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition between CD8 from samples and CD8-HRP conjugate for the anti-CD8 antibody binding site, the intensity of the color is inversely correlated with the concentration of CD8. As more sites are taken up by CD8 from the sample, fewer sites are left that can bind CD8-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration.
Quality Control on Bovine Cluster Of Differentiation 8 ELISA kit
|
Coefficient of Variance |
Intra Variation% ＜10% |
|
|
Inter Variation% ＜12% |
||
|
Recovery |
91-105% |
|
|
Linearity |
Diluent Ratio |
Range % |
|
1:2 |
88-106 |
|
|
1:4 |
87-108 |
|
|
1:8 |
85-110 |
|
|
Specificity/Cross-reactivity |
No significant cross-reactivity or interference between CD8 and analogues was observed. |
|
Send product request
Other supplier products
 |
BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit | E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit Canine Brain Derived Neurotrophic Factor BDNFELISA kitis suitable for the detection ... |
 |
BlueGene Biotech Bovine Decorin ELISA kit | E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of samples from Bovine species. Decorin can also be call... |
 |
Human Asymmetric Dimethylaoyoinine ELISA kit | E01A0035 Human Asymmetric Dimethylaoyoinine ELISA kit Human Asymmetric Dimethylaoyoinine ADMA ELISAkit is suitable for the detection of samples ... |
 |
BlueGene Biotech Porcine Deoxyribonuclease I ELISA kit | E07D0214 Porcine Deoxyribonuclease I ELISA kit The Porcine Deoxyribonuclease I ELISA kit is suitable for the detection of samples from porcine s... |
 |
Rat Active Caspase 3 ELISA kit | E02A0015 Rat Active Caspase 3 ELISA kit Rat caspase 3 elisa kitis suitable for the detection of samples from Rat species. Active caspase 3 can a... |
Same products
 |
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit | Seller: Bluegene Biotech | E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA... |
 |
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit | Seller: Bluegene Biotech | Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E1... |
 |
BlueGene Biotech Chicken Total Adiponectin ELISA kit | Seller: Bluegene Biotech | E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of ch... |
|
|
BlueGene Biotech Bovine Decorin ELISA kit | Seller: Bluegene Biotech | E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of sam... |
 |
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit | Seller: Bluegene Biotech | E11C0339 Bovine C Type Natriuretic PeptideELISA kit Bovine C Type Natriuretic Peptide ELISAkit... |
