BlueGene Biotech Porcine Deoxyribonuclease I ELISA kit
E07D0214 Porcine Deoxyribonuclease I ELISA kit
The Porcine Deoxyribonuclease I ELISA kit is suitable for the detection of samples from porcine species. Deoxyribonuclease I can also be called DNaseI, DNL1, DRNI, DNase-I, Dornase alfa.
Principle of the Assay
Dnase I ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-Dnase I antibody and an Dnase I-HRP conjugate. The assay sample and buffer are incubated together with Dnase I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Dnase I concentration since Dnase I from samples and Dnase I-HRP conjugate compete for the anti-Dnase I antibody binding site. Since the number of sites is limited, as more sites are occupied by Dnase I from the sample, fewer sites are left to bind Dnase I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Dnase I concentration in each sample is interpolated from this standard curve.
As a professional drug residue test kit factory, Shanghai bluegene biotechhas high-quality human insulin elisa kitfor sale. Welcome to contact us if needed.
Send product request
Other supplier products
 |
BlueGene Biotech Canine Ferritin ELISA kit | E08F0010 Canine Ferritin ELISA kit Canine Ferritin ELISAkit is suitable for the detection of samples from Canine species. Ferritin can also be c... |
 |
BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit | E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detecti... |
 |
Expression Plasmids | cre expression plasmidare a kind of double-stranded, closed-loop DNA with a size range from 1kb to more than 200kb. All kinds of antibody expressio... |
 |
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit | E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA kit is suitable for the detection of samples from... |
 |
BlueGene Biotech Porcine Diamine Oxidase ELISA kit | E07D0032 Porcine Diamine Oxidase ELISA kit The Porcine Diamine Oxidase ELISA kit is suitable for detecting samples from porcine species. Diamine... |
Same products
 |
NPK 13-5-40+TE Water-soluble Fertilizer | Seller: Hebei Lvwang Ecological Fertilizer Co., Ltd. | NPK 13-5-40+TE Water-soluble Fertilizer for Vegetables and Fruits NPK 13-5-40+TE water-soluble f... |
 |
China Fertilizer Factory NPK 12-24-12 Compound Fertilizer for Rice | Seller: Hebei Lvwang Ecological Fertilizer Co., Ltd. | China Fertilizer Factory NPK 12-24-12 Compound Fertilizer for Rice NPK 12-24-12 compound Fertili... |
 |
2-Amino-6-chloropurine 10310-21-1 | Seller: Xinxiang Ruicheng Technology Co.,Ltd. | 2-Amino-6-chloropurine 10310-21-1 2-Amino-6-chloropurineis a reactive purine compound used as a ... |
 |
Xanthine 69-89-6 | Seller: Xinxiang Ruicheng Technology Co.,Ltd. | Xanthine 69-89-6 Xanthineis a natural purine base that serves as a key intermediate in purine me... |
 |
Hypoxanthine 68-94-0 | Seller: Xinxiang Ruicheng Technology Co.,Ltd. | Hypoxanthine 68-94-0 Hypoxanthineis a naturally occurring purine derivative involved in nucleoti... |
