BlueGene Biotech Porcine Deoxyribonuclease I ELISA kit

• 

E07D0214 Porcine Deoxyribonuclease I ELISA kit

The Porcine Deoxyribonuclease I ELISA kit is suitable for the detection of samples from porcine species. Deoxyribonuclease I can also be called DNaseI, DNL1, DRNI, DNase-I, Dornase alfa.

Principle of the Assay

Dnase I ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-Dnase I antibody and an Dnase I-HRP conjugate. The assay sample and buffer are incubated together with Dnase I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Dnase I concentration since Dnase I from samples and Dnase I-HRP conjugate compete for the anti-Dnase I antibody binding site. Since the number of sites is limited, as more sites are occupied by Dnase I from the sample, fewer sites are left to bind Dnase I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Dnase I concentration in each sample is interpolated from this standard curve.

As a professional drug residue test kit factory, Shanghai bluegene biotechhas high-quality human insulin elisa kitfor sale. Welcome to contact us if needed.

Другие товары поставщика

	BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit	E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detecti...
	Growth Factor Proteins	Growth factors are a kind of soluble signal molecules, which are necessary to regulate the normal growth and metabolism of microorganisms, but they...
	BlueGene Biotech Chicken Total Adiponectin ELISA kit	E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of chicken elisakits )is suitable for the detection of ...
	BlueGene Biotech Hamster Total Adiponectin ELISA kit	E18A0125 Hamster Total Adiponectin ELISA kit Hamster ELISAkit is suitable for the detection of samples from Hamster species. Total Adiponectin c...
	Rat Active Caspase 3 ELISA kit	E02A0015 Rat Active Caspase 3 ELISA kit Rat caspase 3 elisa kitis suitable for the detection of samples from Rat species. Active caspase 3 can a...

Похожие товары

	порошок ГПМЦ	Продавец: Йида ГПМЦ	Гидроксипропилметилцеллюлоза (ГПМЦ) производится из очищенного хлопка после измельчения, затем ре...
	UHMWPE Plant	Продавец: Hubei Sanli Fengxiang Technology Co., Ltd	Ultra high molecular weight polyethylene (hereinafter called as UHMWPE) is a linear structural po...
	Formic acid production technology	Продавец: Hubei Sanli Fengxiang Technology Co., Ltd	There are totally four production processes forformic acid production, incl. Sodium Formate Proce...
	PPC Plant	Продавец: Hubei Sanli Fengxiang Technology Co., Ltd	PPC (polypropylene carbonate)is acompletely biodegradable environment-friendly plastic synthesize...
	Buy Caluanie Muelear Ship in Europe	Продавец: CALUANIE MUELEAR OXIDIZE	Caluanie (Oxidative Partition Thermostat, Heavy Water) Appearance: Transparent liquid. Smell: Ord...