BlueGene Biotech Porcine Deoxyribonuclease I ELISA kit

• 

E07D0214 Porcine Deoxyribonuclease I ELISA kit

The Porcine Deoxyribonuclease I ELISA kit is suitable for the detection of samples from porcine species. Deoxyribonuclease I can also be called DNaseI, DNL1, DRNI, DNase-I, Dornase alfa.

Principle of the Assay

Dnase I ELISAkit applies the competitive enzyme immunoassay technique utilizing an anti-Dnase I antibody and an Dnase I-HRP conjugate. The assay sample and buffer are incubated together with Dnase I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Dnase I concentration since Dnase I from samples and Dnase I-HRP conjugate compete for the anti-Dnase I antibody binding site. Since the number of sites is limited, as more sites are occupied by Dnase I from the sample, fewer sites are left to bind Dnase I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Dnase I concentration in each sample is interpolated from this standard curve.

As a professional drug residue test kit factory, Shanghai bluegene biotechhas high-quality human insulin elisa kitfor sale. Welcome to contact us if needed.

Другие товары поставщика

	Human Adrenomedullin ELISA kit	E01A0047 Human Adrenomedullin ELISA kit Human Adrenomedullin ELISAkit is suitable for the detection of samples from human species. Adrenomedulli...
	BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit	E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detecti...
	Human Amyloid β Protein 40 ELISA kit	E01A0049 Human Amyloid β Protein 40 ELISA kit Human Adenosine diphosphate ELISA kit is suitable for the detection of samples from human spe...
	Human Adenosine Triphosphate ELISA kit	E01A0038 Human Adenosine Triphosphate ELISA kit Human Adenosine Triphosphate ATP ELISAkit is suitable for the detection of samples from human sp...
	BlueGene Biotech Fish Cyclic Adenosine Monophosphate ELISA kit	E17C0027 Fish Cyclic Adenosine Monophosphate ELISA kit Fish ELISAkit is suitable for the detection of samples from fish species. Cyclic Adenosin...

Похожие товары

	OSi HIOSil BDAC CAS 13170-23-5	Продавец: OSi Holding Limited	Product Description Chemical Name: Di-tert-butoxydicaetoxysilane CAS No.: 13170-23-5 Typical...
	Silicone defoamer, Fatty alcohol defoamer, Polyether defoamer, Mineral oil defoamer	Продавец: ISO certification Food Safety Production License of Anti-foaming agent Halal Certification	Our INVINO antifoams are versatile, catering to a wide range of industries including papermaking,...
	Silicone defoamer, Fatty alcohol defoamer, Polyether defoamer, Mineral oil defoamer	Продавец: ISO certification Food Safety Production License of Anti-foaming agent Halal Certification	Our INVINO antifoams are versatile, catering to a wide range of industries including papermaking,...
	порошок ГПМЦ	Продавец: Йида ГПМЦ	Гидроксипропилметилцеллюлоза (ГПМЦ) производится из очищенного хлопка после измельчения, затем ре...
	UHMWPE Plant	Продавец: Hubei Sanli Fengxiang Technology Co., Ltd	Ultra high molecular weight polyethylene (hereinafter called as UHMWPE) is a linear structural po...