Deoxynuvalenol ELISA test kit

Deoxynivalenol, also known as Vomitoxin belongs to the trichothecene mold streptozotocin family, produced by some Fusarium fungi. Deoxynivalenol are presented in wheat, barley, corn and other grain seeds, the content is usually mg / kg (μg / mL, ppm) level. Because of their high cytotoxic and immunosuppressive properties, Deoxynivalenol constitutes a health threat to human and animal.

This kit is a new generation of fungal toxins detection products in application of ELISA technology, to minimize operating errors and work intensity.

This test kit is based on the competitive enzyme immunoassay for the detection of Deoxynuvalenol in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Deoxynuvalenol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Deoxynuvalenol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Deoxynuvalenol in it. This value is compared to the standard curve and the Deoxynuvalenol concentration is subsequently obtained.

This test kit can be used to detect Deoxynuvalenol in corn and feed s corn, wheat, barley andanimal feedsamples qualitatively and quantitatively.

Sensitivity:10 ppb

Detection limit:

Corn, peanut, wheat and other food crop, feed............................. .200 ppb

Recovery rate:

Beer ………………………………………………………………………..90±10%

Corn, peanut and other food crop, feed ………………………………..95±15%

Wheat, grist ………………………………………………………………..80±10%

Cross-reaction rate

Deoxynuvalenol ……………………………………………………………100%

Coefficient of variation:<10%

1) Micro-well strips: 12 strips with 8 removable wells each

2) 6 x concentrated standards ……………………………………………….1ml each

0 ppb (zero standard), 10 ppb, 20 ppb, 45 ppb, 135 ppb, 405 ppb

3) Enzyme conjugate........................................................................................6ml

4) Antibody working solution.............................................................................6ml

5) Substrate A................................................................ ..................................6ml

6) Substrate B............................................................................................ ......6ml

7) Stop solution.................................................................................................6ml

8) Concentrated washing buffer (10X)..............................................................40ml

9) Concentrated sample diluent (10X)..............................................................40ml

1) Equipment:microtiter plate spectrophotometer (450 nm/630nm), vortex (oscillator optional), centrifuge (funnel optional), balance:0.01gquantity sensitive, gaduated pipettes: 25 ml, polystyrene centrifuge tube: 50m(Triangle bottle with plug optional),Micropipettes: single-channel 20ml ~ 200ml, 200ml ~ 1000ml, multi-channel 250ml

2) Reagents:deionized water

Solution preparation before sample pre-treatment:

1) washing buffer

Dilute the concentrated washing buffer at 1:9 with deionized water to use (1mL concentrated washing buffer + 9 mL deionized water). Solution is stable for 1 month when stored at4 °C.

2) Sample dilute (for food crops and feed)

Take out concentrated sample dilute from 2 ~ 8℃environment, and return to room temperature. If there is a crystal separation, shock, or stirring to make it dissolved then use.

Dilute the concentrated sample diluent (10X) at 1:9 with deionized water to use (1mL concentrated sample dilute + 9 mL deionized water). Solution is stable for 1 month when stored at 4 °C.

Samples Preparation

Instructions (The following points must be dealt with before the pre-treatment)

1)Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;

2)Before the experiment, each experimental utensil must be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results.

3)Store un-handled samples in frozen.

4)After-treatment samples store at 2-8℃in the dark for 24h. All samples should be stored at

2-8℃in the dark.

−Weight enough beer sample and remove CO2.

− Take 50 μl for assay directly.

Dilution factor: 1

Other supplier products

	Deoxynuvalenol ELISA test kit	1. General Deoxynivalenol, also known as Vomitoxin belongs to the trichothecene mold streptozotocin family, produced by some Fusarium fungi. Deoxyn...
	Bovine Brucella Antibody rapid test strip	This test strip is based on fast immuno-chromatography technique to detect Brucella Ab inbovine serum.When testing, Brucella Ab in sample combine w...
	Aflatoxin M1（AFM1）ELISA Test Kit	1. General Aflatoxins are secondary metabolites of the fungi species Aspergillus flavus, parasiticus and nomius. Aflatoxins belong to the strongest...
	Aflatoxins B1 (AFB1) ELISA Test Kit	1. General Aflatoxins are secondary metabolites of the fungi species Aspergillus flavus, parasiticus and nomius. Aflatoxins belong to the strongest...
	Deoxynuvalenol ELISA test kit	Deoxynivalenol, also known as Vomitoxin belongs to the trichothecene mold streptozotocin family, produced by some Fusarium fungi. Deoxynivalenol ar...

All supplier products

Same products

	Services	Seller: Jiang-house Co-operated Co.,Ltd	Bioanalytical Service Bioanalytical Service for Small Molecule Drugs and Metabolites, Large Mole...
	Services	Seller: Hangzhou Ontime I.T. Co., Ltd	Bioanalytical Service Bioanalytical Service for Small Molecule Drugs and Metabolites, Large Mole...
	M-TPU Foam Sheet	Seller: Suzhou Shincell New Material Co.,Ltd.	MTPU is microcellular TPU foam, produced using thermoplastic polyurethane (TPU) as the compositio...
	Adult Manual Toothbrush with Replaced Bristles Russia Version	Seller: Ningbo Maidigg Model and Plastics Co., Ltd	щетка с трех сторон может очистить три стороны зуба, канавы десен и зубной щель, в то же время не...
	Adult Manual Toothbrush with Replaced Bristles	Seller: Ningbo Maidigg Model and Plastics Co., Ltd	щетка с трех сторон может очистить три стороны зуба, канавы десен и зубной щель, в то же время не...