Mouse anti-endothelial cell antibody,AECA ELISA Kit
Thiskitusesenzyme-linkedimmunesorbentassay(ELISA)basedontheBiotindoubleantibodysandwichtechnologytoassaytheHumanGhrelin(GHRL).AddGhrelin(GHRL)tothewells,whicharepre-coatedwithGhrelin(GHRL)monoclonalantibodyandthenincubate.Afterthat,addantiGHRLantibodieslabeledwithbiotintounitewithstreptavidin-HRP,whichformsimmunecomplex.Removeunboundenzymesafterincubationandwashing.AddsubstrateAandB.Thenthesolutionwillturnblueandchangeintoyellowwiththeeffectofacid.TheshadesofsolutionandtheconcentrationofHumanGhrelin(GHRL)arepositivelycorrelated.
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