Human CASP3 (Caspase 3) CLIA Kit

PrincipleThe micro CLIA plate provided in this kit  has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human E-Cad and Avidin-Horseradish  Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human E-Cad, biotinylated detection  antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence  immunoassay analyzer. The RLU value is positively associated with the concentration of Human E-Cad. You can calculate the  concentration of Human E-Cad in the samples by comparing the RLU value of the samples to the standard curve.

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