Human CAMP And CAMP-inhibited CGMP 3',5'-cyclic Phosphodiesterase 10A (PDE10A) E

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Product Name:
Human cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A (PDE10A) E
Product Type :
ELISA Kit
Code :
CSB-EL017681HU
Size :
96T,5×96T,10×96T
Uniprot NO. :
Q9Y233
Abbreviation :
PDE10A
Alias :
PDE10A,FLJ11894, FLJ25677, HSPDE10A, OTTHUMP|dJ416F21.1 (phosphodiesterase 10A)|phosphodiesterase 10A1 (PDE10A1)
Species :
Human
Sample Types :
serum, plasma, tissue homogenates, cell lysates
Detect Range :
125 pg/ml-8000 pg/ml
Sensitivity :
31.25 pg/ml
Assay Time :
1-3h
Sample Volume :
50-100ul
Detection Wavelengt :
450nm
Research Area :
Signal Transduction
BIOWWW :
PDE10A
Precision :
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human PDE10A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 88-97 1:2 Average % 97 Range % 92-102 1:4 Average % 100 Range % 94-107 1:8 Average % 108 Range % 103-114
Recovery :
The recovery of human PDE10A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 102 95-106 EDTA plasma (n=4) 95 89-104
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 8000 1.854 2.012 1.933 1.793 4000 1.466 1.498 1.482 1.342 2000 1.158 1.068 1.113 0.973 1000 0.855 0.856 0.856 0.716 500 0.521 0.535 0.528 0.388 250 0.316 0.326 0.321 0.181 125 0.263 0.251 0.257 0.117 0 0.139 0.140 0.140
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